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We narrowed to 800 results for: cha;

Showing: 461 - 480 of 800 results

  1. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... and forth with. That being said, we probably exchanged dozens of drafts of the manuscript. This is the...transform the document into something cohesive-- change the sentences, make it flow, and start telling ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

  2. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type
    Blog Post
    Published
    July 23, 2019, 12:46 p.m.
    ... PHP.B. The Deverman lab started by seeing how changes in LY6A’s expression affected PHP.B’s transduction...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive...were engineered, check out these two references: Chan, Ken Y., et al. "Engineered AAVs for efficient noninvasive...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ... PAMs, respectively (Walton et al., 2020). Characterization of Cas9 from additional bacterial species ...additional Cas9 species PAM sequence may not be characterized Non-SpCas9's bind a variety of PAM sequences.... Furthermore, non-SpCas9's may have other characteristics that make them more useful than SpCas9. For...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...pre-designed plasmids containing these variants ready for purchase. But what is the easiest and fastest way to use...choose the most effective ones to increase your chances of gene modification in vivo. Find Plasmids for...than the delicate RNA and therefore there has less chance of contamination with RNase leading to degradation...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...FRET has also been used to measure conformational changes, cleavage activity using FRET-based biosensors,...method useful for dynamic experiments in which FRET changes are large. Two other methods are commonly used... such as pH or autofluorescence background, can change the fluorescence decay time and have to be taken...

  6. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ... but sufficiently annotated data used to create charts and tables in the paper are in scope. For images...end” figure or table, clicking on the published chart could reveal the underlying data and allow other...work against transparency. This opens science to charges of elitism and for scientists to be hiding behind...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...blue-light controlled optogenetics, such as channelrhodopsins and tools based on light-oxygen-voltage (LOV...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...developed based on mIFP6. Learn more about the characteristics, performance, and applications of near-infrared...

  8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...First, the imaging of Brainbow mouse tissue was challenging due to low fluorescence intensity, caused partially...fluorescent proteins to find those with ideal characteristics (low aggregation, high photostability, and ...

  9. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...activates/represses gene expression by various mechanisms. The RNA guide is typically 20 nts and the genomic...off-target events Even after you’ve reduced the chance of off-target effects, you still need to do your... knock-out cell line to perform downstream characterization of, isolating 2 or 3 distinct clones to perform...

  10. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...or lab manager be your actual mentor? Is there a chance the supervisor will be moving on soon?   Will you...teaching the stuff you want to know and display character traits and skills that you hope to grow.  Find... you? Maybe you like things a bit hectic and challenging and this drives your best work. Or is it teamwork...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...register and catalyzes their recombination, thereby changing the state of the RSM. If an attB-attP pair is ...general would benefit from a more comprehensive characterization of the recombinases at our disposal.  Many...Vectors Can Be Used for Genome Editing Learn about Michael Koeris' Journey from Grad Student to SynBio Entrepeneur...

  12. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...matrix (usually agarose) to pull your negatively charged DNA through the gel. As indicated in the figure...the gel in wells positioned toward the cathode (- charge). When the voltage is applied across the gel, the... the DNA migrates toward the anode (+ charge). Larger fragments of linearized DNA migrate slower than ...

  13. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... Figure 2. Schematic diagram showing the mechanism of spontaneous reversion during AAV plasmid amplification...phenotype in a mixed population (Burger, C. 2004, Aschauer, DF. 2013, O’Carroll, SJ. 2021). This is due to...outcome.    References and resources References Aschauer DF, Kreuz S, Rumpel S (2013) Analysis of Transduction...

  14. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...quantifies the likelihood that a result is due to chance vs. the factor that is being experimentally manipulated...) or skewed (see Fig. 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test...trial, for example).   Fig. 3: Question flowchart for choosing an ANOVA/MANOVA statistical test ...

  16. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (western blot), IP...BioMolecular Atlas Program (HuBMAP)  Antibody Characterization Laboratory Cancer Any Data repository...

  17. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized...this kit to complement unused auxotrophies in Saccharomyces strains.  Listen to Our Segment on The Yeast...

  18. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions...versions of CRISPR nucleases that make single base changes in the DNA sequence without completely cutting ...

  19. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...(old board games and movies can provide great character costumes). All of this costume planning is for...guess each plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to...

  20. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...dendritic spines!  Curious? Watch our AAV Data Hub Challenge recording to see two reports presented by the ...report describes a functional vector such as a channelrhodopsin Images showing the injection site as well ...
Showing: 461 - 480 of 800 results