We narrowed to 821 results for: nin

...used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK...with the red reporter mRNA. Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes...sequences into the coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these...

... specific tissue type, you mate your fly line containing YFG under the control of the UAS with a fly line...often very easy to get GAL4 UAS experiments up and running with fly lines that are already available, sometimes...references and web links from my first post and start designing your first fly experiment! Many thanks to our...

...publications from these journals into two groups: those containing plasmids that the authors deposited with Addgene... other reasons for the correlation. However, determining this exactly is difficult. For example, it is... papers in the same journals! So, is all this remaining difference caused by depositing the related plasmids...

...mass spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...be beneficial for new researchers or students beginning to learn about biochemistry. The least this app...

...cleave the DNA molecules at specific sites and 2) running the reaction on an agarose gel to determine the...the gel will ensure you keep smaller bands from running off; however, you may need to run the gel for longer...such as how to set up your digests and pouring/running a DNA gel.  More Plasmid Eductional Resources:...

... your academic lab, download Science Careers' "Running Your Lab" booklet. What has been the most surprising...with us every day and in the pub with us in the evening, wanting to know our latest results as soon as ...Resources for Early Career Scientists Science Careers: Running Your Lab (pdf) Advice for Choosing a Research Project...

... pair on actinin will produce different signals based on changes to the structure of actinin in low vs...associated with specific proteins of interest such as actinin. When tension is high vs. low in the cell the donor...

...Replacing the D’ region of an ITR with a sequence containing transcription factor binding sites can increase...expression at varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids...PMID: 31914802. Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact...

...Sanjana et al., 2014). It is a dual-vector system, meaning it is typically used in conjunction with a second...(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD will only be expressed... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several...enzyme. AID, however, only changes cytidines or guanines to the other three bases, which limits the level...bacteria. Its modular nature provides versatility in defining the mutational rate and editing window of the ...

...reached out and asked if I would be interested in joining the audit committee about five years ago. I was...of the office? Katz: I have a puppy and so I am learning how to train a dog with my daughter. It’s super... was associated with Hale & Dorr and Crowell & Morning. Karen was a founding member of Mass Medical Angels...

...across fly lines (i.e. there won’t be positioning effects). When designing experiments where you want to express...process, you will first need to do some molecular cloning. Depending on whether you are trying to overexpress...

...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...display, you can start thinking about your next cloning experiment early on. Beyond these simple but powerful...

... versus eukaryote, background fluorescence). Determining practical brightness When a dish of mammalian...cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there is huge ... under realistic experimental conditions. By determining the brightness for a set of fluorescent proteins...

...are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator...gRNA multiplexing, Xie et al. constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice... gRNA constructs with PTGs. In PTG constructs containing two gRNAs, they found 100% indel frequency at...

...school and turn it into a biotech company. Transitioning an idea from academia to a startup Mike Koeris...competing in business plan competitions. After much learning, the focus of the application was changed, but...million. The biggest challenge during this time was learning to communicate effectively to non-scientific audiences...

... “Learning is not attained by chance, it must be sought for with ardor and attended to with diligence...lookout for friends or colleagues that have the training or skills you want to learn. Mentors can be senior...asking them to have coffee. This is much less threatening! If they say no, don’t worry about it. It is ...

...sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build ...sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein...takes two to three days from the moment you are designing a TALEN on paper to a finished plasmid. With our...

... important to know all your options when planning and running experiments. You never know what problems...in an ~50kD protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; ...

...particularly interested in whether institutions planning to raise salaries prior to the injunction are ...injunction (see below). Of the institutions who were planning to raise salaries before the injunction, we have... contacted by a number of postdocs who are now planning unionization at their institutions. One clear ...