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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 817 results for: nin

Showing: 461 - 480 of 817 results

  1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ... a only a single gRNA per cell. The viral DNA containing the gRNA then integrates into the genome. Thus...are poorly transduced by lentivirus, and thus screening with available CRISPR libraries was not possible...retroviral mouse CRISPR pooled library can be used for screening of cells that are refractory to lentiviral transduction...

  2. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...fluorescent proteins and a new two-photon laser scanning microscope will allow Tsakanova to image cortical...insights into functional network interactions. Combining synthetic biology with engineering: glowing bacteria... bacterial sensor, he will construct plasmids containing promoter regions that activate fluorescent protein...

  3. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...mass spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...be beneficial for new researchers or students beginning to learn about biochemistry. The least this app...

  4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...cleave the DNA molecules at specific sites and 2) running the reaction on an agarose gel to determine the...the gel will ensure you keep smaller bands from running off; however, you may need to run the gel for longer...such as how to set up your digests and pouring/running a DNA gel.  More Plasmid Eductional Resources:...

  5. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ... your academic lab, download Science Careers' "Running Your Lab" booklet. What has been the most surprising...with us every day and in the pub with us in the evening, wanting to know our latest results as soon as ...Resources for Early Career Scientists Science Careers: Running Your Lab (pdf) Advice for Choosing a Research Project...

  6. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several...enzyme. AID, however, only changes cytidines or guanines to the other three bases, which limits the level...bacteria. Its modular nature provides versatility in defining the mutational rate and editing window of the ...

  8. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...display, you can start thinking about your next cloning experiment early on. Beyond these simple but powerful...

  9. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... versus eukaryote, background fluorescence). Determining practical brightness When a dish of mammalian...cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there is huge ... under realistic experimental conditions. By determining the brightness for a set of fluorescent proteins...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ... pair on actinin will produce different signals based on changes to the structure of actinin in low vs...associated with specific proteins of interest such as actinin. When tension is high vs. low in the cell the donor...

  11. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...reached out and asked if I would be interested in joining the audit committee about five years ago. I was...of the office? Katz: I have a puppy and so I am learning how to train a dog with my daughter. It’s super... was associated with Hale & Dorr and Crowell & Morning. Karen was a founding member of Mass Medical Angels...

  12. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Sanjana et al., 2014). It is a dual-vector system, meaning it is typically used in conjunction with a second...(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD will only be expressed... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...are commonly packaged in 400-500 bp cassettes containing the RNA pol III promoter, gRNA and pol III terminator...gRNA multiplexing, Xie et al. constructed PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice... gRNA constructs with PTGs. In PTG constructs containing two gRNAs, they found 100% indel frequency at...

  14. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...school and turn it into a biotech company. Transitioning an idea from academia to a startup Mike Koeris...competing in business plan competitions. After much learning, the focus of the application was changed, but...million. The biggest challenge during this time was learning to communicate effectively to non-scientific audiences...

  15. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... “Learning is not attained by chance, it must be sought for with ardor and attended to with diligence...lookout for friends or colleagues that have the training or skills you want to learn. Mentors can be senior...asking them to have coffee. This is much less threatening! If they say no, don’t worry about it. It is ...

  16. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...sequence? Musunuru: The kit includes 832 plasmids containing prefabricated pieces that allow you to build ...sequence in the genome in rapid fashion. The two remaining plasmids in the kit have the rest of the protein...takes two to three days from the moment you are designing a TALEN on paper to a finished plasmid. With our...

  17. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    Published
    Feb. 28, 2019, 1:34 p.m.
    ...this pivotal moment that Teytelman realized that running interactive workshops to train researchers on tools...expanding this team, leading monthly calls, and planning next steps. April Clyburne-Sherin had already ...there, allowing others to have the resources for running their own workshops: April Clyburne-Sherin’s and...

  18. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ... power (Shaner et al, 2008). In confocal laser scanning microscopy, fluorescent proteins are excited with...photon-excitation, selective plane illumination, TIRF or spinning disk confocal use entirely different excitation...rates can be summarized as follows: Instead of determining a t½ of a fluorescent protein in a water/oil ...

  19. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... important to know all your options when planning and running experiments. You never know what problems...in an ~50kD protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; ...

  20. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...particularly interested in whether institutions planning to raise salaries prior to the injunction are ...injunction (see below). Of the institutions who were planning to raise salaries before the injunction, we have... contacted by a number of postdocs who are now planning unionization at their institutions. One clear ...
Showing: 461 - 480 of 817 results