We narrowed to 695 results for: prot

...degradosome, which contains enzymes that cut RNA not protected by secondary structure. It is thought that poly...complex and involves two RNA polymerase-associated proteins, CPSF and CstF, which are responsible for recruiting... tail is important for stability of the mRNA, protection from degradation, and is integral to the nuclear...

... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...Number of fragments assembled Golden Gate Assembly protocol* (Volume of 1mL Outgrowth plated) Correct assemblies...

...machinery into diverse bacteria species. Existing protocols can transfer CRISPRi into a single bacterial strain...resistance selection markers, gRNAs, and dCas9 proteins. Second, it can be introduced into a broader range...selection by TnsD, a sequence-specific DNA binding protein. Selection removes donor bacteria and recipient...

... process, check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and...process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process...Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR...

...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014...

...are distributed under the terms of the UBMTA to protect our depositors’ IP, but there may also be extra...as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can find additional... by the depositor including genbank files and protocols here. Articles Citing this Plasmid: This is a ...

...whole mouse brains expressing green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories... Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace...Tomer, L. Ye, B. Hsueh, K. Deisseroth. (2014) Nat Protoc., 9, 1682-1697. Pubmed. C. Soderblom, D.-H. Lee...

...error. Used in combination with standard barrier protocols, they can reduce the risk of accidental escape...research have conclusively shown that barrier protocols are vulnerable to human error. And with gene drives...original sequence, it can restore the original protein-coding sequence using a recoding strategy; a subsequent...

...vectors simultaneously express two or more separate proteins from the same mRNA. We've discussed promoters ...site, thereby resulting in co-expression of two proteins from a single mRNA. IRES was originally discovered...your gene of interest along with a fluorescent protein or selectable marker, it is easiest to start with...

...mutations result in an inactive RNA-guided DNA binding protein (dCas9). dCas9 is then tethered to a repressive...MS2 RNA aptamers, and the  MS2-P65-HSF1 helper protein (Konermann et al., 2015). Root and Doench also ...2018): 356626. 6. Tanenbaum, Marvin E., et al. "A protein-tagging system for signal amplification in gene...

...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...

...express a red, green, or blue (RGB) fluorescent protein (4a-c). When high doses of this viral cocktail ...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...-use AAV from Addgene here or check out these protocols if you want to package your own virus. ...

...mutation, so you don’t accidentally change your protein of interest.   Fig. 2: Stop re-cutting ...further, you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase,...used to prime a Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9...

...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...

...that couldn’t package itself due to a few missing proteins. The envelope plasmid contained a viral envelope...for viral production: Gag (structural precursor protein), Pol (polymerase), Tat (viral transactivator for...export of transcripts). A different viral envelope protein (Env), usually from VSV-G due to wide infectivity...

...plasmids/viruses are needed to deliver all the protein and RNA components, and several sgRNAs must be ... start sites, all of which yield the same BDNF protein. To demonstrate the specificity that can be achieved...inserted human CD8, a gene that encodes a surface protein for which there are good flow cytometric antibodies...

...DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the nanopore...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...

...keeping. Top priorities in most cases include IP protection, publications and funding. If any of these three...need share data with them including reports and protocols. To facilitate this, some ELNs allow you to invite...witnessing and audit trails. How does the ELN provider protect and respect your IP rights and data ownership? ...

...social contract citizens enter into in order to protect their rights (2). Less formally, governments are...for a given policy. In exchange, the government protects (and often funds [3]) scientists as well as other...Aeronautics and Space Agency, the Environmental Protection Agency, and the National Institutes of Health...

... BirA, has been used to identify and study protein-protein interactions in proximity labeling techniques...extensive research history and characterization. Many proteins and processes naturally found in E. coli have ...