We narrowed to 640 results for: son

1. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ... O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang...

2. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

   Type

   Blog Post

   Published

   Oct. 3, 2017, 12:11 p.m.

   ...This series was written for selfish reasons: I wanted to learn about careers in science communication...

3. Some Like it Hot: Thermostable GeoCas9

   Type

   Blog Post

   Published

   Sept. 14, 2017, 12:40 p.m.

   ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...

4. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals...

5. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

   Type

   Blog Post

   Published

   June 11, 2019, 1:16 p.m.

   ...it from anywhere. Its ease of use is one of the reasons why this was the first program I used to create...

6. Cellosaurus: A Cell Line Information Database

   Type

   Blog Post

   Published

   Feb. 21, 2023, 2:15 p.m.

   ...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

7. Hot Plasmids and Viral Preps - July 2021

   Type

   Blog Post

   Published

   July 27, 2021, 1:15 p.m.

   ...off-target effects.   New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

8. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ...protocol to remove endonucleases activity. For these reasons choosing the correct solution is fundamental, and...

9. Plasmids 101: What is a plasmid?

   Type

   Blog Post

   Published

   April 2, 2020, 6:17 p.m.

   ...restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately chosen ...

10. Bringing Sustainable Practices to the Lab: Recycling

    Type

    Blog Post

    Published

    Feb. 14, 2019, 2:35 p.m.

    ...easy to spot and even easier to ship back. Be that person! If you use serological pipettes from Corning...

11. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ...question is: how should the gRNAs be oriented in comparison to each other? The gRNAs must target different...

12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    Published

    Feb. 13, 2018, 2:51 p.m.

    ...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...

13. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type

    Blog Post

    Published

    Dec. 13, 2017, 3:20 p.m.

    ...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

14. Tips for Improving Your Next Manuscript

    Type

    Blog Post

    Published

    Nov. 22, 2016, 3:30 p.m.

    ...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

15. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...tips for applying it in your own lab. Table 1: Comparison Between Standard Restriction Cloning and Fast...

16. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type

    Blog Post

    Published

    July 2, 2015, 8:37 p.m.

    ...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

17. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type

    Blog Post

    Published

    Jan. 15, 2015, 1:50 p.m.

    ...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

18. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    Published

    Aug. 19, 2014, 3:39 p.m.

    ... Lianna Swanson...

19. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

20. History of CRISPR Cas - A tale of survival and evolution

    Type

    Blog Post

    Published

    Nov. 25, 2013, 10:12 p.m.

    ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...