We narrowed to 842 results for: Eras

... developed, though, researchers at HuidaGene Therapeutics guessed that fusing MPG to an ABE would allow...transversion editor AYBEv3. This tool still retained considerable A → G editing activity at most sites, in addition...they co-expressed Polη, a translesion synthesis polymerase known to preferentially incorporate A bases across... N108Q mutation in the deaminase), produced an average A → C product purity of 48%. Like CGBEs, these ...abasic site before filling the empty space. Over several rounds of rational mutagenesis, the team progressed...allow it to recognize thymine (T) or cytosine (C). Several different teams succeeded with this approach around...recognize T and/or C substrates. The HuidaGene Therapeutics team has deposited their editors, called gTBE...

...preps and our lentiviral preps.   Viruses are generated using standard methods that have been optimized...optimized for each specific virus in order to generate high quality preparations. After production, all virus...the University of Pennsylvania Vector Core. In general, titering is performed by the facility that produced...suggests that titers obtained using ddPCR are generally two to three fold higher than those achieved using... us. Full sequencing of the Viral Genome Next-generation sequencing is performed on viral genomes isolated...items) are produced in 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed ...lentiviral preparations (“-LVC” catalog items) are generated from lentiviral preparations (described above)...

...pEcCas/pEcgRNA is superior in several ways: The pEcCas plasmid can be operated at 37 ℃ for faster growth ...plasmid that expresses the S. pyogenes Cas9, a temperature-sensitive replicon for self-curing, an arabinose..., 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB gene...containing BsaI restriction enzyme sites at its ends to generate pEcgRNA. The ccdB serves as a counterselection...genes of interest in BL21(DE3) using the newly generated pEcCas/pEcgRNA system.   Advantages of the pEcCas...directly ligated with the annealed 24 nt oligos to generate new pEcgRNA. pEcCas/pEcgRNA can be applied not... the gene dosage of glucose dehydrogenase and generated a strain that could achieve glucose dehydrogenase...

...processes from development to metabolism to fertility. Several zebrafish-specific gene expression databases that...available, new hypotheses about specific genetic interactions arise and tools to deactivate specific genes...way to deactivate genes without spending months generating mutants. Unexpectedly, phenotypes sometimes differ...of gene regulation (El-Brolosy et al., 2019). Generating zebrafish transgenics Creating transgenic organisms...different experimental conditions. Strategies for generating transgenic zebrafish are well established (Felker...cell RNA sequencing approaches (Harland, 2018). Several different methods have been developed, relying ...i.e., redundant) genome, their long three-month generation time and labor-intensive maintenance compared...

...more stable than the methyltransferase. When a plasmid is lost, the methyltransferase is degraded and/or...stabilized luciferase-expressing plasmids to examine in vivo-induced promoters in the Vibrio cholerae vaccine...ensure that plasmids are passed down through generations (Figure 1). These so called “plasmid addiction...composed of restriction endonucleases and methyltransferases. Restriction endonucleases cleave DNA at ...specific nucleotide sequences. To counter this, methyltransferases add methyl groups to bases at specific nucleotide...stably express antigens in sufficient quantities to generate an immune response. As a proof of concept, they...genes that couple host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences...

...fall back to? We have outlined some important considerations to help you choose the plasmid prep kit that...kit to use?  Obtaining higher quantities of DNA generally means starting with higher volumes of bacterial...reduce your ultimate plasmid yield.  Take your average column-based miniprep kit for example. These kits...buffers reduces plastic waste from bottles and the overall weight of the package during shipping. Of the kits...optimized based on the plasmid size, cell model, or overall throughput needed. Large constructs – Large plasmids...medium-throughput applications. For high-throughput options, several manufacturers offer automation-friendly kits and...here!) Rapid Isolation – If you’re in a hurry, several manufacturers also offer kits with speedy protocols...

... replicates how you’d interact with team members in person, and improves overall communication.  Maintain...project and accelerates the pace at which problems can be addressed and new ideas generated. Furthermore...team in Paris, France. While efforts like these accelerate the progress of research, they can also present...different tasks. But these challenges can be exaggerated when communicating across time zones, digitally...the times that work best for them. This also accelerates the pace of research by circumventing the slower...exchange project at the International Cancer Immunotherapy Conference in Frankfurt, Germany When funding...

...Virus Genome Interactions Important for Vector Production and Transduction. Human Gene Therapy 31:499–511...and Rep40) Rep proteins. Alternative splicing generates the different C-terminal regions of the Rep proteins...transfer plasmid, which contain regions of dsDNA. To generate a 3’ hydroxyl group needed for DNA replication...in the ITRs, uses its endonuclease activity to generates a ssDNA substrate which is then nicked by its ...splicing and differing translational start sites generate the three VP proteins. The three VP proteins assemble...Adeno-Associated Virus (AAV) as a Vector for Gene Therapy. BioDrugs 31:317–334 . https://doi.org/10.1007/...Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https...

...genome engineering - such as certain forms of gene therapy - avoiding the use of viruses is also important...lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts resulted in...approximately 100-fold more efficient than the first-generation transposase (as determined by transposition assays...construct. This new tool kit allows the rapid generation of stable transgenic cell lines which can robustly... CD19-directed chimeric antigen receptor (CAR) therapy. SB is a valuable tool and an alternative to CRISPRs... Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Biotechnol J. 2015...Mates L, et al. Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable...

...guide RNAs may tolerate a mismatch in the binding sequence, we recommend changing several bases as close... to as ‘indels’. While this is convenient for generating gene knockouts, NHEJ repair does not allow introduction...introduction of specific sequence changes. To generate a specific sequence change the cell must undergo...other groups have focused on strategies to improve overall HDR rates, we have shown recently that desired ...desired genome-editing events by HDR can be generated more efficiently by (1) improving editing accuracy by ...systematically characterize this phenomenon and found a general relationship between the distance of a mutation...probabilities our data revealed, and found that for generating a homozygous HDR event with optimal efficiency...