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Showing: 721 - 750 of 885 results
  1. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...your data by Nov. 12 to compete. The top ten participants will be invited to compete in our speed round...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...
  2. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...
  3. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...more commonly known as fruit flies, have been an important animal model for research, particularly genetics...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...
  4. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...tube-labeling, quick plate pouring, and much more. We didn’t want to keep the knowledge we’ve gained along the way...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...
  5. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...worth of ladder. The Penn State ladders are significantly cheaper. When using a homemade plasmid prep ...increases the costs to $20-30 per ladder: still significantly cheaper than commercial ladders. Are you interested...
  6. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    Published
    Feb. 9, 2017, 3:30 p.m.
    ...story of Rosalind Franklin and her incredibly important role in solving the structure of DNA. 2. The...her or her family's knowledge. 3. Fossil Rock Anthem One of Tom's earliest and most popular videos. ...
  7. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene. These are... critical systems that cannot be removed or substantially reduced in an attempt to save energy. The transition...
  8. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for...for the Addgene blog simply email us. We can’t guarantee that all those who sign up will be able to write...
  9. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    Published
    Dec. 2, 2021, 2:15 p.m.
    ...that you can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science... festivities - however you celebrate! And if you want to see how Addgene celebrates and decorates, follow...
  10. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...in CRISPR including: Base editing Prime editing Anti-CRISPRs … you name it! So get excited and download...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...
  11. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ... focusing on safety and the ability to generate mutant microbial strains faster than ever before. 29:54... end : How Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution of new...
  12. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    Published
    Jan. 23, 2018, 2:04 p.m.
    ...References 1. Choi, Vivian W., et al. "Production of Recombinant Adeno‐Associated Viral Vectors for In Vitro and...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...
  13. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...  has seen a fantastic response from the research community. Customers have taken advantage of the customizability...
  14. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...search bar Now, you can search for the articles you want to find without even leaving the Addgene blog! Just...articles on the Addgene blog by subscribing to instant alerts below.  ...
  15. Make a Splash: Notions of Scientific Impact Are Evolving

    Type
    Blog Post
    Published
    Dec. 5, 2014, 2:54 p.m.
    ...toiling away in laboratories this holiday season want the work you are doing to have an impact, to move...libraries and offering another way to consider and quantify the impact of scientific publications. Ventures...
  16. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...expression level without the need for a custom antibody. There is one major caveat - a tag may interfere...affinity for other proteins, and accessibility to an antibody. From their initial pool of candidates, they selected...
  17. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type
    Blog Post
    Published
    June 26, 2014, 2:23 p.m.
    ...week. So I have developed some good habits that I wanted to pass on to you to make sure you are getting ... you properly visualize your results.The most important of those is to always look closely at the trace...
  18. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 7, 2017, 1:58 p.m.
    ...bioluminescent, some types of corals and anemones are vibrantly colored. He hypothesized that GFP-like proteins...they’re used, see some new techniques that take advantage of them, and give some tips on deciding which ...
  19. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...accomplishments. Employers and academic departments want to be able to tell that you know about something...Learned Sooner by Peggy Klaus Looking for a mentor or want to start a peer mentoring group? "What Makes a ...
  20. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... they’ve engineered SECURE adenine base editor variants using a protein-truncation strategy with reduced...Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity and decreased...
  21. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...choosing a specific serotype, but occasionally you may want to further control where your gene of interest is... many more tips and ideas on how to use these fantastic tools in your experiments.  Many thanks to Hongkui...
  22. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...and see if we should start celebrating papers and grants too with pizza or pub.” The Cepko Lab celebrates...required to achieve success in the lab, it is important to take the occasional breather and acknowledge...
  23. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...Organize you experiments, keep track of upcoming grant deadlines, and sync everything to your calendar.... updates to protocols in real time. You may also want to consider moving your paper spreadsheets for reagent...
  24. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...double strand breaks. Researchers have long taken advantage of this natural process to integrate protein tags...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...
  25. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...physical scientists are underrepresented. It’s important to note that these results could be biased for...Resources Check out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist...
  26. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...Scholars summer program. I was unsure whether or not I wanted to major in biochemistry. There, I had exchanged...has changed my life for the better, and I wouldn’t want it any other way. Toj Toj-Villagran is a rising ...
Showing: 721 - 750 of 885 results