We narrowed to 750 results for: Tro;

1. Transferable Skills Guide: Managing a Team

   Type

   Blog Post

   Published

   Jan. 30, 2018, 2:42 p.m.

   ...mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences but ...

2. Hot Plasmids: Winter 2025

   Type

   Blog Post

   Published

   March 11, 2025, 1:15 p.m.

   ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

3. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

4. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...expression. We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo...

5. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

   Type

   Blog Post

   Published

   Oct. 11, 2018, 12:30 p.m.

   ...using similarly sized Lambda amplicon inserts to control for any suppression of background by antibiotic...

6. Plasmids 101: 5 factors to help you choose the right cloning method

   Type

   Blog Post

   Published

   Aug. 21, 2018, 12:31 p.m.

   ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...

7. Plasmids 101: Optimizing Plasmid Yields

   Type

   Blog Post

   Published

   May 26, 2016, 2:30 p.m.

   ... Digest Additional Resources Sigma-Aldrich Introduction to Microbial Media BitesizeBio: How to Get Better...

8. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

9. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ... with a good public school system that has made strong efforts in the last 5 years to incorporate more...

10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type

    Blog Post

    Published

    Oct. 10, 2023, 1 p.m.

    ...  Susanna Stroik...

11. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ... messy, and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the...

12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ... requires people to be in groups, organize your troops prior to the event, and let people know exactly...

13. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ...application. They have increased their demand for stronger budgets with clear budget narratives, logic models...

14. To Codon Optimize or Not: That is the Question

    Type

    Blog Post

    Published

    Nov. 12, 2020, 2:15 p.m.

    ...from GenBank database (CUTG), that provides an electronic dataset for codon-usage of 257,468 genes across...

15. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...SHERLOCK had a few limitations when it was first introduced in 2017 so the lab made further modifications...

16. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The team originally...

17. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    Published

    March 12, 2019, 1:37 p.m.

    ...Medical Institute. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

18. How to Be an Excellent Trainee

    Type

    Blog Post

    Published

    Sept. 26, 2023, 1:15 p.m.

    ... Susanna Stroik...

19. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    Published

    Dec. 13, 2016, 3:30 p.m.

    ...recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban Asbestos Now Act of 2016...

20. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...expressed genes (Emilsson and Kurland, 1990). Controlling gene expression through gene sequence - Proteins...

21. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ...onto data longer before publishing. But there are strong incentives to publish such as precedence, recognition...

22. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...2018). NIR FPs can be combined with blue-light controlled optogenetics, such as channelrhodopsins and tools...

23. March for Science

    Type

    Blog Post

    Published

    April 21, 2017, 2:30 p.m.

    ...Additionally, interest groups are often pandered to when strong or overlooked when small, but data is not going...

24. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...Antibodies 101: Flow Cytometry Antibodies 101: Introduction to Antibodies...

25. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type

    Blog Post

    Published

    Oct. 11, 2016, 2:30 p.m.

    ...Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab.  As with all plasmids in the repository, we...

26. How to Negotiate a Successful Lab Start Up Budget

    Type

    Blog Post

    Published

    Sept. 1, 2015, 2:30 p.m.

    ...excessive costs in your lab. That in itself is a strong negotiating tactic, and puts you ahead of most ...

27. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type

    Blog Post

    Published

    Nov. 6, 2015, 3:30 p.m.

    ...work? IMP Pilot Program 2015-2015 Scholars need strong proactive mentors who have relevant expertise and...

28. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...effects. Typically the phenotype would be studied in vitro, but there is enormous potential in performing in...

29. Cancer and the Immune System: Deciphering the Relationship

    Type

    Blog Post

    Published

    March 14, 2017, 2:30 p.m.

    ...reduction as well as progression. Dunn et al in 2002, introduced the “Three Es of Immunoediting” nomenclature....

30. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ..., well understood, and the first taught when introducing CRISPR as a technology. There are three subtypes...