We narrowed to 554 results for: ESP
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TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit...
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Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....colleagues who read it with me, the consistent response from scientist and non-scientist alike was that... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....you to lose soluble proteins from the sample, especially if left on for too long or used at too high a... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....variability. Moving forward It took a five-team response to address this issue, but it still points to ... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....life cycle. One should ponder this figure often, especially the three steps from the 12 o’clock to the 6 ... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....accurate measure of DNA concentration. This is especially problematic in low-concentration samples (samples... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m.....1371/journal.pone.0007750 Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....mono-methylation, tri-methylation, and aceylation, respectively (these are chemical modifications, or functional... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise that researchers... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....you used for cloning. This type of control is especially important for insert-specific primers. If your... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....beta-lactam resistant population to the antibiotic, despite this resistance gene being encoded by a high-copy... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....Karl-Klaus Conzelmann. "Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....post-translationally modified, co-expression of the enzyme responsible for the modification in the assay host strain... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....holding the tubes and use this ID to name the corresponding Excel sheet.3. Be specific - When assigning... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....consultant to faculty writing research grants, especially in the sciences and by extension to scientifically... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m.... in the wildtype protein and facilitating its widespread use in research. Many other mutations have since... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....risk. Biosafety should always be considered with respect to the specific experiments being performed and... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....nonfunctional proteins and protein fragments respectively. Solving the problem of codon usage bias - codon... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....recognized or leaky in Agrobacterium. This is especially problematic with reporter genes as you might ...