We narrowed to 566 results for: FIE

1. Cloning Mammalian Cells with the Agarose Method

   Type

   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ...form a firm monolayer. The cell clones - easily identified from the plate circling- are then individually...

2. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

   Type

   Blog Post

   Published

   July 13, 2017, 2:30 p.m.

   ...more resources that may be more tailored to your field of study. 5 great resources to help get your fly...

3. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

   Published

   June 22, 2017, 2:30 p.m.

   ...proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported in vesicles ...

4. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   Published

   May 4, 2017, 2:30 p.m.

   ...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...

5. Tips to Make the Most of a Scientific Conference

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   Blog Post

   Published

   May 23, 2019, 12:26 p.m.

   ...have additional questions. If you or your lab identifies a potential collaborator from the meeting, reach...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   Published

   Feb. 7, 2019, 2:16 p.m.

   ... guide and the DNA target. CFD scores are then modified (“transformed”) such that weak interactions (e.g...

7. Pooled Library Amplifications

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   Blog Post

   Published

   May 30, 2024, 1:30 p.m.

   ...be useful to run a restriction digest of your amplified library on a gel to ensure that your conditions...

8. Plasmids 101: Degron Tags

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   Blog Post

   Published

   March 14, 2024, 1:15 p.m.

   ...Degrons are destabilizing domains which will be identified by various enzymes and sent to the proteasome...

9. Plasmids 101: Knockout/Knock-In Plasmids

   Type

   Blog Post

   Published

   Dec. 1, 2016, 3:30 p.m.

   ...The concept is simple: deliver a template with a modified version of the targeted sequence to the cell which...

10. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...

11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins...

12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...early 2000s, a handful of chromoproteins were identified in sea anemone (Chan et al., 2006). Why do these...

13. Experimenting with New Careers while in Grad School

    Type

    Blog Post

    Published

    Feb. 25, 2016, 3:30 p.m.

    ...Your Research Learn About A Variety of Different Fields on Our Collections Pages  ...

14. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...drives? Then do so! It's a tremendously promising field that could address many pressing global problems...

15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...

16. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...innovative solutions necessary for advancing the field. References: 1. Internal initiation of translation...

17. Tips for Using BLAST to Verify Plasmids

    Type

    Blog Post

    Published

    May 29, 2014, 1:29 p.m.

    ... your plasmids instead! All BLAST images are modified screen shots from the NCBI BLAST website. Additional...

18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    Published

    Sept. 8, 2016, 2:30 p.m.

    ... Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule...

19. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome...

20. CRISPR Antimicrobials

    Type

    Blog Post

    Published

    May 3, 2016, 2:30 p.m.

    ...fashion with the introduction of antibiotics, but the field is currently undergoing a renaissance. Yosef et ...