We narrowed to 608 results for: Sis;
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TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...
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The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,... -
Predicting Adverse Reactions to Monoclonal Antibody Drugs
TypeBlog PostPublishedFeb. 13, 2024, 2:15 p.m....3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole... -
K. phaffii: Rising to the Occasion
TypeBlog PostPublishedApril 4, 2023, 1 p.m....the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....found, to providing reagents in an accessible and consistent way so other researchers can use them, to making... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog PostPublishedDec. 20, 2018, 1:15 p.m....report is that when it became clear that this assistive reproductive technique was feasible, three separate... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m.... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m....current and future features being built for the analysis software program, and meet with the automation... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog PostPublishedMay 19, 2020, 1:15 p.m....2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog PostPublishedJan. 8, 2019, 1:54 p.m....customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m.... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....validate your new construct by Sanger sequence analysis. But for this research, it was likely that the... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....data in a manner that demands high accuracy and consistency? If the answer to any of these questions is... -
Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA. Similar to a jigsaw...