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  1. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  2. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...

  3. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...therapeutic delivery by AAV, which has strict capacity limitations. New efforts have recently been made to increase...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

  4. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ...at this event added to a growing dialogue on the state of a system that, despite all of its successes, ...Andrew Young School of Policy Studies at Georgia State University, is an expert on the academic labour ...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...the take rate of mouse xenografts after cell implantation. Their goal was to figure out how to optimize...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

  6. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ... that flank their DNA fragment in an inverse orientation. This allows for multiple DNA components (promoters...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

  8. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...sense to get my master’s and leave. I was really devastated when that happened. I didn't know if I should.... The choice to not go to medical school was devastating. At the same time, she has an entirely new path...

  9. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...MuLE (Multiple Lentiviral Expression) system facilitates the simultaneous introduction of multiple genetic...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...paper, but positive outcomes can include poster presentations, talks about your work, or even the successful...Learn How to Become a Grant Writing Professional Facilitate Career Planning and Personal Development with...

  11. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...scientific articles, patents, and even some doctoral dissertations. Streamline the question asking process Having...answer, consider asking to avoid unrealistic expectations. And finally, provide feedback. If you are...

  12. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...However, many fluorescent proteins face similar limitations, and many labs are already using these chromoproteins...promoter. The resolution step leads to the desired mutation and excision of the vector backbone. To do this...

  13. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...industry, you’re more likely to give an oral presentation to highlight any new data or ask for additional...industry with most team members giving oral presentations, you will also have to attend many more meetings...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...due to its endA1 recA1 relA1 genotype, as these mutations improve plasmid stability and yield. If a plasmid...using older plates could increase plasmid loss or mutation. Let it incubate Increasing the incubation time...

  15. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...for CINSO. For instance, I tweeted to the Indiana State Museum and others when I was trying to find local...informal science educators, and ever since, the State Museum has been the best supporter and most productive...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ..., tobacco, rice, sugarcane, and even tubers of potatoes have been used for protein expression. Plant ...well-defined media and can be easily adapted to fermentation allowing for large-scale, stable production ...

  17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ... Base editing, another method to create point mutations, also has a strict editing window about 15 nt ...mutagenesis plasmid (MP6) permits quick, large-scale mutation of the gene of interest. They created a selection...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...deleting a problematic copy of a gene that causes a debilitating disease! This is, of course, only hypothetical...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K, D540M) that shows... and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M, in response...

  20. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... that was not “poised for cleavage”. Jacob computationally added the non-template strand to the Jinek ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...
Showing: 481 - 500 of 705 results