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We narrowed to 715 results for: lic

Showing: 481 - 500 of 715 results

  1. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey...

  3. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  4. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ... membranes is tricky because they have fewer hydrophilic surfaces, require detergents or lipids to remain...2020) Generation of synthetic nanobodies against delicate proteins. Nature Protocols 15:1707–1741. https...

  6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...

  8. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  10. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...containing smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts...

  11. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...

  12. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  15. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...mitochondrial MDH (hMDH2) with those from human cytosolic MDH (hMDH1). They then expressed the original ...

  16. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...missing so that we can make a new tube from a replicate sample. The transformation After the DNA has ...plasmid can grow. As the bacteria divide, they replicate and retain the plasmid. This allows us to make...

  17. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...protein evolution By Joanne Kamens Mateo Sanchez and Alice Ting have used one biotechnology tool to optimize...cultured and FACS sorted. Image from Mateo Sanchez and Alice Ting. Sanchez MI et al., Nat methods. 2019. https...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...submitted to Addgene have minor differences due to in silico variations and lack of verification. To address...Nanopore sequencing technology, bioinformatics and applications. Nature Biotechnology, 39(11), 1348–1365. https...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...community is no small task. The EDGE program solicitation requires funded labs to rapidly disseminate ...dissemination of information and materials. Many groups publicized workshops on their organismal systems for scientists...
Showing: 481 - 500 of 715 results