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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: nin

Showing: 481 - 500 of 816 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...and many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-Cre...only in specified cells or at specified times. Combining this with some of the loxP methods described above...

  2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...intracellular Ca2+ levels than the original version containing CRY2 without the extra peptide. CRY2clust should...Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic operon that enables the degradation...enzyme required for conversion of xanthine into guanine, and without xanthine supplied in the media, these...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...underlying genetic causes of some human conditions, opening up new avenues for therapeutics that treat disease...immunological reconstitution with a normally functioning T cell repertoire (1). In this example, it was...Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous...

  4. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions with Vivid (Vvd) photodimers... protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated cell sorting...s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic Observation and Tagging...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...fluorescence from other regions of the cell. By combining vectors expressing different fluorescent proteins...can be used to determine cell cycle length by examining the ratio of blue to red cells. Cells undergoing...Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express...

  6. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ... working in the pharmaceuticals industry after earning my Master’s in 2016 and then working four more ...to get enough sleep the night before. Have your morning coffee, if that’s part of your daily routine. Stay...s Science Career Guide Check out Addgene’s job openings  ...

  7. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...expression in the retina.  Four approaches for designing synthetic promoters  To build a library of synthetic...marker (CatCH-GFP). The lab deposited AAV plasmids containing the active promoters at Addgene. Performance ...expression occurred in one type of cell, with the remaining expression occurring in only Müller glia cells...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...are used as it eliminates the need for antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ... How Lentivirus is Used in CRISPR Genome-Wide Screening Learn How Lentivirus Has Been Used for Lineage...

  10. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ...content. Here are some of my tips to organizing and running a memorable panel discussion. Check out Joanne'...where the questions are obvious, the answers uninteresting, the panel members all say the same thing and...will thank you. Own it. For more great tips on running panel discussions see Guidelines for Great Panels...

  11. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... of DNA through error-prone non-homologous end joining (NHEJ). In targeted DNA knock-in, donor DNA is ...CRISPR-Cas delivery utilized the Non Homologous End Joining (NHEJ) DNA repair mechanism to knock out genes ...https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current...

  12. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    Published
    July 2, 2020, 12:40 p.m.
    ...and gRNAs. Using lentiviral delivery of the two remaining CAPTURE 2.0 components eliminates the need to ...identified and the level of on-target enrichment. 2. Determining the spatial and hierarchical organization of ...Variant for Your Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional...

  14. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding...gene names in the figure below to find plasmids containing that particular gene. ...colocalization experiments in a scientific and meaningful way it is important to choose the appropriate...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome...studies of animal development where events like the patterning of nascent tissues and the formation of limbs... process. How have you used FP timers? Are you planning on using an FP timer in a new and creative way...

  16. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ... unstained sample for each tissue type you are running in your panel if you wish to use the autofluorescence...indistinguishable. This tool is of particular value when designing a panel.     Figure 3: Similarity index...if markers are co-expressed in your cells when designing your panel. If you have two highly overlapping...

  17. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ...soon developed that allowed researchers to create stunning, interactive images of their proteins. Since the... known to say something silly like “See there, arginine 53 really is right there, at the end of helix ...description of how effective this model was in explaining CRISPR technology to 4th graders, check out Jennifer...

  18. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...depend on what downstream applications you are planning, cost, and the amount of protein you need to produce...popular choice for this. Chromatography columns containing appropriate immobilized ligands are incubated...incubated with cell culture supernatant containing antibodies. The antibodies bind the immobilized ligands and...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...retroviruses are RNA-containing viruses  (Robinson et al., 1965)! You may be beginning to realize that the...Lander, M. R., Pugh, W. E., & Teich, N. (1971). Noninfectious ARK mouse embryo cell lines in which each cell...

  20. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...continue such discussions. Beyond the excitement of learning about new cutting edge science, researchers of...the most important is letting people know it’s happening. Aim for 3-4 weeks advance notice for the first...presenter, figure by figure) if the primary purpose is training graduate students in critical reading. If the ...
Showing: 481 - 500 of 816 results