We narrowed to 695 results for: prot

...help prevent the accumulation of such truncated proteins. Examples of use:  Ghosh et al., 2008 used a...donor A may be capable of expressing a truncated protein. This can be avoided by excluding a stop codon ...AAV vectors with reduced expression of truncated proteins are safe and effective in the retina of a mouse...

...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell... Distinguishing correctly folded and misfolded proteins is essential for human health. Failure to do so...

...very minimum requires an Argonaute protein. This Argonaute protein is what binds the small RNA and either...cleaves the target mRNA or recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA...

...the application of this approach is limited to protoplast cells (plant cells whose cell wall has been removed...many of the specifics - promoters used, precise protein sequences or domains, and methods of delivery -...recommend reading the associated publications or protocols to get the most out of the plasmid you chose for...

...primarily by frameshift mutations in dystrophin, a protein essential for proper muscle function. Without functioning...are multiple common deletions that maintain the protein’s reading frame, leading to the production of a ...a smaller, but at least partially functional protein. Individuals with these mutations are often asymptomatic...

... AAVs “unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and...receptor (AAVR)2   AAV2 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR; not strictly required.../α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan (HSPG)3 AAV receptor (AAVR)2 Epidermal growth...

...bacterial strain that is deficient in recombinase protein RecA can reduce the frequency of multimerization...circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20... Biology ReferenceAddgene's Restriction Digest Protocol More resources on the Addgene blog:  Plasmids ...

...of secretory proteins in the Endoplasmic Reticulum, optimization of fluorescent proteins,  and live cell...actually answer your question? If you're using a new protocol or reagents, do you understand all of the steps...

...Resources on Addgene.org Basic Molecular Biology Protocols Protocol Videos Molecular Biology Reference Pages  ... critical reagents for many molecular biology protocols, including vector-insert joining for recombinant...

... pluripotent stem cells from urine samples. Nat Protoc, 2012. 7(12): p. 2080-9. PubMed PMID: 23138349....from human ES and patient-specific iPS cells. Nat Protoc, 2015. 10(7): p. 941-58. PubMed PMID: 26042384....cerebral cortex neurons and neural networks. Nat Protoc, 2012. 7(10): p. 1836-46. PubMed PMID: 22976355...

...somewhat useful for the latest NTT targeting Cas protein, Cpf1. Separately, on-target efficacy is still ...but the good news is that you only need one. ProtospacerWB was developed with the purpose of applying CRISPR..., online), DESKGEN (commercial, online), or ProtospacerWB (academic, offline). While it remains to be ...

...launch of our partnership with the Institute for Protein Innovation (IPI). Together, we’ll be working to... blog, including our antibody guide, antibody protocols, and Antibodies 101 blog series.  Stay tuned for...

...learn about optogenetics tools for controlling protein activity, methods for increasing CRISPR editing...this segment Hot Plasmid 1: Photocleaveable PhoCl protein for controlling molecular processes with light....

...System, or FLIS, incorporates a bioluminescence protein into the chitin that can be activated only by a... a specific frequency. Once activated, the protein emits a brief yet bright burst of light, visible to...

...and Jillian Parker who published their work on a protein required for cytokinesis in JEI. Developing an ... at Addgene.org Find basic molecular biology protocols Check out our molecular biology reference pages...

...to create the most informative and entertaining protocol and explainer videos for you to level up in the... This could be you after watching Addgene’s protocol videos. via GIPHY   If you’re also looking...

...Resources on Addgene.org Check out Our Protocol Pages for New Protocol Videos View Our Molecular Biology Reference...

...tools useful to any molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral...? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells   ...and many more! Sign ...

...Cepko's recent work on developing GFP-activated proteins, read our recent blog post. Check out the break...al. “A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation...

...to cut other DNA or RNA sequences. Nucleases: Proteins that bind to gRNAs and are directed by the gRNAs...prime editing and the identification of other Cas proteins continue to expand the targeting range. CRISPR...