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TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m....she will start her own research group at Boyce Tompson Institute. References Baldwin, Melinda. "Credibility...
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Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m.... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m.... Rachel Leeson... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....stop codons. New from the viral service By Jason Nasse We regularly add new viral aliquots from our... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....recombination in samples. For a more detailed comparison of these cloning strains, browse the table of... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....they exist but are unreliable for a variety of reasons (Baker, Nature). Tagging your protein allows you... -
Antibodies 101: Chimeric Antibodies
TypeBlog PostPublishedApril 25, 2023, 1:15 p.m.... Morelock, M. M., Rothlein, R., Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R.,... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....the gut-brain axis (admittedly for self-serving reasons – I want to understand my obsession with the combination... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m.... Rachel Leeson... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m....strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... light. Inspired by @UriAlonWeizmann, here my song about our paper recently published in @nchembio ... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....mismatches or indels. Empirical tests indicated that a reasonable rule is to require at least 96% sequence identity... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m.... Lianna Swanson... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....shift won’t happen overnight, but there are a few reasons why you might choose NgAgo over CRISPR proteins... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....to add overhangs to your insert of interest for Gibson assembly. The steps following primer design and... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....functionality of the RNA scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward...