We narrowed to 842 results for: Eras

...through the proliferation of specific, and not all, Langerhans cells, to create foci of proliferating cells....Brainbow The Lichtman group developed the original iteration of this technology in 2007. Used to label neurons... is expressed. Cre-mediated deletion of one or several of these genes permits the expression of genes ...occurs. Following the development of Brainbow 1.0, several more refined versions have been developed, which...tracing in zebrafish with Zebrabow Before the generation of Zebrabow (Pan et al., 2011), Brainbow was ...Tissue-specific Cre recombination in this mouse generates cells that randomly express either nuclear GFP...become a powerful tool for understanding cell proliferation. Inducible XFP expression in the Ubow mouse ...

...edited. For many experiments, the end goal is to generate a clonal cell line, so it’s important to verify... for a needle in a haystack before going on to generate and screen individual clones. If a clonal line...al., NAR). This technique uses the trace file generated in Sanger sequencing and a simple algorithm to...with your sgRNA sequence. The TIDE software will generate a graph representing all insertions and deletions... 4 clones. Did you use a dual guide system to generate your knockout? Then you’re in luck! You don’t ... to visualize an edit. Most dual guide systems generate a deletion large enough that it can be visualized...pair edit with a prime editor. What next? When generating a knock-in mutation, the best screening method...

...which chews back the 5’ ends of the fragment, generating long overhangs which allows the single stranded...stranded regions with homology to anneal a polymerase, to fill in the gaps a DNA ligase, to seal the nicks...enzymes is that they can all work at the same temperature, so the entire reaction takes an hour or less...advantage is that this process makes it easy to generate wild type and mutant constructs at the same time...an overlap of 15-40 bp, with a primer melting temperature greater than 48℃. Both Snapgene and NEB have ...instead of using the exonuclease feature of T4 DNA polymerase, which must be controlled by the presence or ...methods that have been described--CPEC (circular polymerase extension cloning) and SLiCE (Seamless Ligation...

...Over the past several months, we’ve seen the emergence of several SARS-CoV-2 variants coincide with increased...the world” says Balazs. “By combining those with [sera] we got from people that have been given the vaccine...relative ability to neutralize.”   Figure 2: Sera used in the study came from individuals who received... two doses of the Pfizer or Modern vaccine. This sera was used in the high-throughput SARS-CoV-2 pseudovirus.... In their assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection...Figure 3: Neutralization outcomes of convalescent sera with various pseudotyped virus. Image from Garcia-Beltran...quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research. “I hope that by sharing...

...important consideration is whether the affinity of an experimental protein-ligand interaction approaches...non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized on... target protein with the vitamin biotin. The interaction between biotin and the egg-white protein avidin...affinity (i.e. sub-nanomolar) protein-ligand interactions. Avidin and its derivatives have four biotin-binding...involves amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids...solution was to exploit the best of both worlds. We generated a plasmid that produces biotin ligase and a protein...erythropoietin receptor and subsequently evaluated interactions with mutant and wild-type erythropoietin molecules...

...we believe it is best to further reduce onsite operations for the coming week. In an abundance of caution...Addgene charges enough for our services to run our operations. We don’t raise money except occasionally for...to launch a new service or product. Our daily operations for all ongoing processes are entirely self-supporting...they grasped the crucial role Addgene plays in accelerating research. Yet this was still not enough to ensure...funding organizations to request support for our operations until our normal work resumed. Addgene distributes...type of biomedical science research. Our work accelerates and enhances productive collaborations as well...distribution of plasmids for COVID-19 research is accelerating essential research in laboratories all around...

...The contents of this post are intended to convey general information only and not to provide legal advice...you think about your career (and your future in general), keep in mind that you may have years to work ...for recent Ph.D. graduates). Therefore, it is imperative to plan for and develop your career into a series...Order which sets broad policy intentions directing federal agencies to propose reforms to the H-1B visa system...collaboration management, grant administration, clinical operations, and so on. Candidates should look broadly at...and your continued work is necessary to create therapeutics to cure the cancer. Authorship:  Publishing ...Interest of the EB-1A. Furthermore, the ability to leverage the prestige of the employer makes EB-1Bs a very...

... to have its genome fully sequenced There are several human disease models established in yeast, including... new at all, but it’s not as well-known in the general research community. Phillips Petroleum Company...release the K. phaffii expression system to the general research community in the 1990s when they abandoned...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...Thus, the production of glycoproteins for human therapies, such as antibodies, is possible in K. phaffii...peroxisome studies since their peroxisomes will proliferate upon a media change into methanol and peroxisomal...peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation K. phaffii’s genome has...

...scientific community. Recently, the Zhang lab generated a TF library (aka the MORF Collection) and an ...isoforms transcribed from these sites. The Zhang lab generated a comprehensive library of each unique TF open... representing 92% of the MORF library, with an average of 206 cells per TF ORF. From this large sequencing...map of possible TF-induced cellular states was generated (Joung et al., 2023). Fig. 1: Barcoded ...then compared to that of reference cell types generated by the human fetal expression atlas. Computational... Good disease models can often be difficult to generate, in part because of difficulty with replicating...and a program that any researcher could use to generate the relevant disease model. Accurate modeling ...

... is the Arabidopsis thaliana - Pantoea agglomerans interaction. But the shape and metabolism of leaves...anticoagulant drug warfarin. In past research, several VKOR variants have been found that affect the body...postdoctoral work in Lin Tian’s lab at UC Davis is to generate a genetically encoded fluorescent sensor for serotonin...but these drugs are often ineffective or require several rounds of dose and drug adjustments. Unger ultimately...to study plant-bacteria and bacteria-bacteria interactions  Michal Bernach is a graduate student in Mitja...University of Canterbury where he studies how bacteria interact with each other and plant surfaces. His system...leaves can be changed by light and temperature and leaf autofluorescence can interfere with microscopy. Thus...