We narrowed to 554 results for: ESP

1. New Tools Enable CRISPRa for Neuroscience Applications

   Type

   Blog Post

   Published

   July 2, 2019, 12:51 p.m.

   ...neurotrophic factor (BDNF). Bdnf is expressed early in response to neuronal activity (hence, “immediate-early”...

2. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...questions can derail your talk. You need to balance respectfully answering questions while steering the conversation...

3. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ... to which she was applying. I probably did not respond to her question as well then as I would now. I ...

4. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...deaminate cytosine placed in 2-6 bp and 4-8 bp, respectively, of the protospacer. And if the target cytosine...

5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...Cells Induces Gamma Rhythm and Controls Sensory Responses.” Nature 459(7247): 663–67. PubMed PMID: 19396156...

6. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...’ end of  the tRNA binds the amino acid that corresponds to the anticodon sequence. That means there are...

7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively (Walton et al., 2020). Characterization of Cas9...

8. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...References  del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control...

9. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ... slightly higher, than those of agarose beads, despite the size difference. Magnetic beads are particularly...

10. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...

11. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...scaffold to map the reads, which boosts ‌accuracy, especially in repeated regions. If this doesn’t resolve ...

12. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your...

13. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments because yeast tend...

14. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    ...of pTargetF into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated...

15. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...chunks, we have the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another...

16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...in sufficient quantities to generate an immune response. As a proof of concept, they tested a lux-mediated...

17. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...However, the Sendai virus method is expensive, especially if used to make clinical grade hiPSCs. For example...

18. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...Specialist, Technical Support Advisor, Technical Correspondent or similar. While there isn’t a clear distinction...

19. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    Published

    April 7, 2016, 2:30 p.m.

    ...titer virus is used, it is not always feasible, especially when the lentiviral stock needs to be titrated...

20. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References...