We narrowed to 557 results for: ESP

1. Designing Your Chalk Talk for the Academic Job Interview

   Type

   Blog Post

   Published

   March 12, 2019, 1:37 p.m.

   ...questions can derail your talk. You need to balance respectfully answering questions while steering the conversation...

2. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ...in sufficient quantities to generate an immune response. As a proof of concept, they tested a lux-mediated...

3. Plasmids 101: Codon usage bias

   Type

   Blog Post

   Published

   Sept. 27, 2018, 1:09 p.m.

   ...nonfunctional proteins and protein fragments respectively. Solving the problem of codon usage bias - codon...

4. Analyzing CRISPR Editing Results with ICE from Synthego

   Type

   Blog Post

   Published

   May 8, 2018, 1 p.m.

   ...sample by clicking on the sample name or on its corresponding bar graph entry. Clicking to initiate the in-depth...

5. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

   Type

   Blog Post

   Published

   Oct. 19, 2017, 1:54 p.m.

   ...Cells Induces Gamma Rhythm and Controls Sensory Responses.” Nature 459(7247): 663–67. PubMed PMID: 19396156...

6. To Codon Optimize or Not: That is the Question

   Type

   Blog Post

   Published

   Nov. 12, 2020, 2:15 p.m.

   ...’ end of  the tRNA binds the amino acid that corresponds to the anticodon sequence. That means there are...

7. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...SpRY, can target NGN PAMs and NRN and NYN PAMs, respectively (Walton et al., 2020). Characterization of Cas9...

8. Antibodies 101: Immunoprecipitation

   Type

   Blog Post

   Published

   Dec. 7, 2021, 2:15 p.m.

   ... slightly higher, than those of agarose beads, despite the size difference. Magnetic beads are particularly...

9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

   Type

   Blog Post

   Published

   Sept. 9, 2025, 1:15 p.m.

   ...letter following the enhancer ID (h, m, or q, respectively). These sequences were then cloned into an AAV...

10. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...scaffold to map the reads, which boosts ‌accuracy, especially in repeated regions. If this doesn’t resolve ...

11. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...easy-to-understand guided manner. These videos can be especially useful when designing new primers to clone your...

12. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...cerevisiae and E. coli. Auxotrophic selection is especially common in yeast experiments because yeast tend...

13. Hot Plasmids - November 2023

    Type

    Blog Post

    Published

    Nov. 14, 2023, 3:35 a.m.

    ...-iPAK4 expressed under promoters like the tet-responsive element (TRE) or the cFos promoter. For example...

14. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    Published

    Dec. 14, 2017, 2:08 p.m.

    ...However, the Sendai virus method is expensive, especially if used to make clinical grade hiPSCs. For example...

15. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...Specialist, Technical Support Advisor, Technical Correspondent or similar. While there isn’t a clear distinction...

16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    ...of pTargetF into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated...

17. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...References  del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication and Control...

18. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...chunks, we have the flexibility to adapt and easily respond to changing needs of the teams at Addgene. Another...

19. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ... to which she was applying. I probably did not respond to her question as well then as I would now. I ...

20. CRISPR-mediated Plant Base Editors

    Type

    Blog Post

    Published

    Jan. 3, 2019, 1:35 p.m.

    ...deaminate cytosine placed in 2-6 bp and 4-8 bp, respectively, of the protospacer. And if the target cytosine...