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We narrowed to 836 results for: Eras

Showing: 501 - 520 of 836 results

  1. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods towards...smaller than 10uL. You can leave them at room temperature when you’re working with them, but avoid freeze-thaw...okay, Blugene!    Buffers  You know what temperature to store your antibodies at, but it also matters...commercial kits.  However, antibodies conjugated to horseradish protein (HRP), primarily used in Western blots...

  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...study microbiome interactions with Alzheimer's, I found others using CRISPR to generate new knockout mice...within the biological sciences, there’s plenty of interaction between neuroscience and other fields. For instance...us produce new educational content on the blog. Overall, there are many exciting things going on in the...

  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...content - the useful information on scientific literature, technical skills, and experiences from thousands... Nearly everything we write entails a primary literature search to collect up to date information on the...review process led to quick dives back into the literature to make sure absolutely everything was correct... typos, and hit publish. Making content is an iterative process. We are constantly reviewing the resources...

  4. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...yeast, are temperature sensitive screens. These involve raising or lowering the culture temperature to exacerbate...drug-containing media in which only cells which can tolerate the drug survive and everything else dies off....though. A selection can take the form of varied temperature, nutrient availability, drugs, and much more....

  5. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...interest by PCR generates mutated plasmids with no methylation (there are no methyltransferases in the PCR ...DNA. The other component of these systems, methyltransferases, methylate DNA at particular sequences to...restriction modification systems containing methyltransferases that add methyl groups to specific DNA sequences...

  6. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...to do a massive RNA-seq analysis. I had never interacted with a computer at the command line, and so was...keep track of the files that I was continuously generating.  I quickly found that there is no one way to...physical lab notebook, along with any figures generated from the analyses: Goal: Before you begin, briefly...control your commonly used scripts: If you edit a general purpose script for a single use, save the script...

  7. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ... in the human genome. The pair has focused considerable attention on transcription factors, starting...Myers: Our labs work really closely together on several projects and this is one related to gene expression...There’s also a question about how specific those interactions are. This is solved in this CRISPR system with...Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9 in the lab ...

  8. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Broad Institute's Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with... off,” he said. “Some are strong and some more moderate; there is an allelic series where the degree to... use in various cell types and in live mice. Therapeutic uses in humans are also on the table. “In the...with the libraries will require access to next generation sequencing technology. These libraries are ...

  9. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...technology and giving consumers control over their interaction with it, we hope to inspire and open a new conversation...synthetic biology In addition to public resistance, an overall limited understanding of plant biology is a significant... genetic pieces developed over the years were generated by large agricultural companies and carry associated...biotechnology is full of potential, but there are several hazards to navigate when mapping out a new project...

  10. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...alternative reagents are created in the lab, instead of generated in vivo, they have higher reproducibility and ... Fig. 1: A comparison of an antibody (left) to several alternative affinity reagents.    Types of ...small enough to be produced in bacteria and are generally cheaper to make than antibodies. Minibodies are...instead of the antibody constant region. They are generated using combinatorial libraries, and high specificity...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibodies work?  Secondary antibodies are antibodies generated against antibody isotypes from a specific species...classes, it is possible to get secondary antibodies generated against isotype subclasses, such as mouse IgG2...interest - the secondary antibody needs careful consideration before selection. If all the primary antibodies...my goat anti-rabbit?!”*  *a secondary antibody generated in goat against rabbit immunoglobulins. References...

  13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...phenotypes. Factors such as the growth medium, growth temperature, and growth rate can all impact plasmid copy ... as the incoherent feedforward loop (iFFL). In general, iFFLs use an input signal that both positively...repression in E. coli. In addition, TALEs bind non-cooperatively to the promoter region, which is predicted to... pathway will ideally be optimized to promote generation of the desired product while avoiding the accumulation...

  14. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ... would do if she wasn’t at the bench.  “I was literally sitting in my office one day, which I shared with...NEI), where she wrote about NEI research for a general audience. She then got a full time position with..., however, recommend using Twitter as a way to leverage unpaid work for paid work: “If you use it right...helped because there was so much work through the federal government. All the Health and Human Services (...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...there’s less malignancy because there’s less proliferation. However, the lack of caveolin 1 in breast cancer...tumor, proving once again that a tumor is always interacting with its surroundings and being shaped by them...growth mediators and improving cancer cells proliferation.  How do microenvironments affect tumor spread...regulates transcription. Without TKA1, cells proliferate and cannot undergo apoptosis, thus multiplying...

  16. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...cultured with isotope-labeled amino acids for several hours to several days, followed by a short period of growth...via the secretory pathway, and then cleaved to generate an active form of the protein. To monitor autophagy...Pho8Δ60 in the vacuole, where it is cleaved to generate an active enzyme. Pho8 phosphatase activity or...

  17. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    .../feudal generating too many trainees per PI” “Lack of awareness about how the system operates and functions...issues independently; for example, Harvard’s Therapeutics Graduate Program has a compulsory internship...better data is needed to help shape and inform the overall structure of the biomedical research sector, and...

  18. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...abundance and clonal dynamics over time using next generation sequencing (NGS). The library was developed when...such that initial resistant populations can be eradicated up-front quickly. This approach is in contrast... discovery and the development of combination therapies.         References 1. Hyo-eun, C. Bhang, et al...Studying clonal dynamics in response to cancer therapy using high-complexity barcoding." Nature medicine...

  19. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...collaborates with these companies to create and provide general availability of their recombinant antibodies. Since...that the primary antibody is very weak or with general non-specific binding. If the sample differs from...align with what the atlases (mentioned above) and literature suggest, providing an expectation and comparison...imaged pattern does NOT align with the atlases/literature and is unrelated to the target protein, we categorize...

  20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ... its analysis can be found here: A Novel Next-Generation Sequencing and Analysis Platform to Assess the...the pool - ex. the gRNA in a CRISPR library. In general, our QC scientists work with the depositing lab...Mueller I, Mitsopoulos A, Fan M (2020) A Novel Next-Generation Sequencing and Analysis Platform to Assess the...Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum.2019.277...
Showing: 501 - 520 of 836 results