We narrowed to 566 results for: FIE
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TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....)00023-6. PMID: 12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses...
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CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m..... An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms. Nat Chem... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....with the role our repository has played in these fields, and seeing names like Feng Zhang, Jennifer Doudna... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....Addgene homepage. You may have noticed the search field front and center. Let’s type in “crispr” and see... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....Genome engineering, and many other technologies and fields in biology, are moving so quickly that it’s basically... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....TK, Krishnappa N, Sachdeva R, Deutschbauer AM, Banfield JF, Doudna JA (2020) Targeted Genome Editing of... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....reduced chance for off-target effects. As the CRISPR field continues to evolve, it is likely that the use of... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... Fluorogen activating proteins (FAPs) – These modified antibody fragments bind and enhance small molecule... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....insert-specific primers. If your negative control amplifies a product of the expected size, you know the genome... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....fashion with the introduction of antibiotics, but the field is currently undergoing a renaissance. Yosef et ... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....tube’s appropriate location. The barcode also identifies the contents of each individual tube. If a plate... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....that rut! We’ve done everything from kayaking, to field day, to relay triathlons, to 5Ks. Many of these ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m.... active. Further, GFP can label transgenically modified ES cells, which can then be used for implantation... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....be at every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog PostPublishedDec. 13, 2016, 3:30 p.m....such as these has brought a lot of promise to a field that has historically been full of bad news and ... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m.... This construct can be easily expressed and purified from E. coli, retains important functional properties... -
Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....Cre-expressing vector resulted in a sparsely-labeled field of view that was more desirable than having many... -
Antibodies 101: Reading a Flow Plot
TypeBlog PostPublishedFeb. 6, 2024, 3:13 p.m.... population of interest has been successfully identified and isolated. Following a gating strategy Figure... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....efforts beyond your organization and/or scientific field. Reaching out to people who have little in common... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m....In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand in the way of creative...