We narrowed to 610 results for: REV
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TypeBlog PostPublishedAug. 5, 2021, 12:15 p.m....(PV)-expressing neurons, neurons that the lab previously found had a role in perceiving touch inputs. ...
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Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....stay away from polyclonal antibodies The most prevalent issue with polyclonal antibodies is their batch... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m.... - the tech transfer community. In a recent law review article we received some entirely unsolicited praise... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....Person.” If that happens a few times, LinkedIn may revoke your ability to send invitations. Finally, if you... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....fragments and you will remove the buffers used in the previous reactions. This will increase your chances to ... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....germline editing is permissible in cases of disease prevention. However, it is important to consider that already-tested... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....their own unique features and quirks. Here, we will review the genome engineering abilities of the lesser-... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m....increase stability. Nature has evolved ways to prevent degradation of important cellular RNA molecules... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....for this purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....formation of the desired ligation product is irreversible because this construct does not retain the enzyme... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....suggestions. Most coaches have websites you can review and most create useful online content in the form... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....regulatory molecules, and has provided clues to their previously uncharacterized cellular roles. In the current... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m....Potential Applications in Biotechnology. Critical Reviews in Biotechnology, 24(4), 155–208. https://doi.org... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits ... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....and PAM-flexible nucleases! In this blog, we will review Cas enzymes, which have a flexible or non-existent... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....(NHP) brains. Systemic gene delivery capsids previously identified in rodents have not been widely successful... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog PostPublishedJune 25, 2020, 1:15 p.m....when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me pursue this, let ... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....nucleus-targeted GCaMP6 with an extra apoCaM-binding motif to prevent aberrant binding of the biosensor to endogenous... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m..../364/6436/eaav5282.long Redfern CH, Coward P, Degtyarev MY, Lee EK, Kwa AT, Hennighausen L, Bujard H, ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....choice affect my experiment? In this post we will review some of the main factors that affect isotype choice...