We narrowed to 524 results for: SHE

1. Five Popular Model Organisms, Part 2

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   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...with their regenerative abilities is expected to shed light on human aging pathological developments (...

2. Transferable Skills Guide: Conflict Resolution

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   Blog Post

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   Jan. 4, 2022, 2:15 p.m.

   ...want, but that means the other party will feel punished or invalidated. Both compromise and arbitration...

3. Antibodies 101: Introduction to Immunofluorescence

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   Blog Post

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   Nov. 16, 2021, 2:15 p.m.

   ...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

4. Plasmids 101: Expressing shRNAs for RNA Interference

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   Blog Post

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   June 17, 2025, 1:15 p.m.

   ... Alyssa Shepard...

5. Hot Plasmids: Summer 2024

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   Blog Post

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   Aug. 27, 2024, 1:15 p.m.

   ..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...

6. CRISPR 101: Homology Directed Repair

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   Blog Post

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   Jan. 26, 2023, 2:15 p.m.

   ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

8. Generating Mouse Models Using CRISPR/Cas9

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   Blog Post

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   July 12, 2016, 2:30 p.m.

   ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

9. Plasmids 101: Restriction Cloning

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   Blog Post

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   Feb. 18, 2016, 3:42 p.m.

   ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

10. CRISPR 101: Epigenetics and Editing the Epigenome

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    Blog Post

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    June 24, 2020, 5:45 p.m.

    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

11. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

12. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post

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    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

13. Quick Guide to All Things Lentivirus

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    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

14. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

15. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

16. Custom CRISPR Screens & the Green Listed Software

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    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

17. Antibodies 101: Introduction to Antibodies

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    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

18. Antibodies 101: Flow Cytometry

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    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

19. Typing CRISPR Systems

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    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... Alyssa Shepard...

20. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...the many primary research papers that have been published at this point.  In brief, some standard opsins...