We narrowed to 526 results for: SHE

... Alyssa Shepard...

..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...

... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

... Alyssa Shepard...

... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...