We narrowed to 526 results for: SHE

1. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...reliable in the future.  We hope that this post has shed some light on immunofluorescence. The technique ...

2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

   Type

   Blog Post

   Published

   Dec. 2, 2019, 2 p.m.

   ...molecules per cell volume? A call to rethink some published values." Bioessays 35.12 (2013): 1050-1055. PubMed...

3. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   Published

   Aug. 15, 2019, 12:30 p.m.

   ... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...

4. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

5. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...with their regenerative abilities is expected to shed light on human aging pathological developments (...

6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

7. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   Published

   Jan. 26, 2023, 2:15 p.m.

   ...resulting in off-target effects. A technique that pushes the size limitations of ssODNs is Easi-CRISPR (...

8. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

   Type

   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

9. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

10. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

11. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

12. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

13. Plasmids 101: Restriction Cloning

    Type

    Blog Post

    Published

    Feb. 18, 2016, 3:42 p.m.

    ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

14. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

15. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

16. Quick Guide to All Things Lentivirus

    Type

    Blog Post

    Published

    March 21, 2017, 2:30 p.m.

    ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

17. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... Alyssa Shepard...

18. Antibodies 101: Flow Cytometry

    Type

    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

20. Antibodies 101: Introduction to Antibodies

    Type

    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...