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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 844 results for: STI

Showing: 501 - 520 of 844 results

  1. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...an important feature of a fluorophore. The key question is how the brightness is best defined or determined... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...

  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...

  3. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and its potential applications. Challenges of existing mutagenesis methods Several continuous diversification...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your targeted...

  4. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...the open science movement is that it provides interesting and unique challenges for intellectual property...that balance is, and will continue to be, an interesting legal and professional challenge.Paratore: The...executive (CHIE). He is a member of both the Internal Institute of Auditors and the Information Systems Audit ...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ... This method can work well, but poor template digestion can result in a high number of background colonies...fragment In vitro Assembly No No Yes Yes DpnI Digestion Yes Yes No No Purified Enzymes Required No No...

  6. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...have something in common. Namely, the scientific question you are interested in and this is a great ice ...relationships. Make a list of experimental problems or questions you are pondering and be ready to talk about them...scientists your own age. Some will be friendly so stick with those. Don’t miss social opportunities, but...

  7. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...? Sometimes, the funders of your research may stipulate that your article must be published in an open...scope of the journal you are sending it to. It’s wasting everybody’s time if you don’t. Add a clear cover...get writing! Let us know if you have any other questions about writing and publishing your manuscript, ...

  8. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...discussion with resources. One of the most common questions I get is, “What are we going to talk about?” There...internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings. Experiment...ideas from your own mentoring group or with any questions about mentoring. Read the other posts in the series...

  9. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...- These fragments are produced by proteolytic digestion that retains an antibody’s disulfide bonds within...resulting product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - ...- These fragments are produced by proteolytic digestion above the hinge-region, which results in two independent...

  10. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...target tissue, two weeks is the standard ballpark estimation. Once expressed, viral cargo is usually quite...a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal circuits of the retina using viral ...12349826. Xiong, Wenjun, and Constance Cepko. "Distinct expression patterns of AAV8 vectors with broadly...

  11. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ... to make a plasmid. Doench’s lab in the Broad Institute has created hundreds of thousands of plasmids,...also a great way to get to know people doing interesting and relevant work in your field! However, there...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

  12. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...tet-off turns off constitutively expressed proteins while tet-on turns on constitutively silenced proteins...know what the job requirements are. Here are the questions you should be asking. What kind of control? On...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...initiates single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only...that it generates staggered breaks, also known as sticky ends. Cas12a cleavage results in 5 bp overhangs...efficient, multiplexable RNA knockdown. Cas13 has also stirred up interest for its potential as a viral therapeutic...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...there and what are they doing. To address these questions, biologists often use next-generation sequencing...microbiota and host biomarkers In clinical and diagnostic labs, deep sequencing and qPCR methods are too...Jeffrey Barrick’s labs at The University of Texas at Austin recently developed the Bee Microbiome Toolkit (...

  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...core mission. Plasmid distribution is amazingly still growing at >9% every year (12% in 2018) since 2005... The viral vector team is publishing research, hosting conferences, and are expanding our catalog of materials...community of scientists who support us by depositing, requesting and spreading the word. Our blog and site ...

  16. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ... admittedly, somewhat meaningless) practice of listing “Submitted” articles in their CV or grant applications...directly alongside the preprint while the work is still in progress or awaiting a final publication in a...Motivations, concerns and selection biases when posting preprints: a survey of bioRxiv authors. bioRxiv...

  17. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...this though) about the fact that containers are distinct from their contents. You should be tracking both...tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would...getting up and running and more time working on interesting biotechnology code.There are valid technical ...

  18. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded lentiviral...pool of lentiviral plasmids. Multiple rounds of digestion and ligation result in plasmid libraries containing...tailored to address the users’ specific research questions. CombiGEM has been successfully applied to functionally...

  19. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem ...adult stem cells isolated from two patients with cystic fibrosis (Schwank et al). They were able to demonstrate...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ... instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic system To set...they retain their intracellular interactions and still set off the same pathways, giving rise to the same... create ultrapotent PSEMS (uPSEMs) that have drastically increased potency. Download our chemogenetics...
Showing: 501 - 520 of 844 results