We narrowed to 609 results for: cas
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TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....be molecular biologists. This was not at all the case. The conference featured people who work in sociology...
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Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot like a game of telephone... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....Transformation of Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m.... EGFP and mEGFP. Many of these features, such as Cas9, weren’t detected by our in-house software. In fact... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m.... endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal for imaging ... -
Plasmids 101: Screens vs. Selections
TypeBlog PostPublishedNov. 10, 2022, 2:15 p.m.... Genetic screens in human cells using the CRISPR/Cas9 system. Science, 343(6166): 80-84 (2014). doi: https... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources on Addgene.org... -
Sweating the Small Stuff: Details in the Lab
TypeBlog PostPublishedFeb. 8, 2022, 2:15 p.m....before, which got rid of the issue entirely. In many cases, the answer isn't to work harder - it's to work ... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....sodium azide at all, as it inhibits HRP. In those cases, thimerosal at a concentration of 0.01% w/v can ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....that share homology in the same reaction. In these cases, the use of another cloning method, such as Golden... -
Viral Vectors 101: Types of viruses
TypeBlog PostPublishedJune 6, 2023, 1:15 p.m....large enough for many applications, but in some cases multiple AAVs (co-infection), an alternative virus... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....representations of the 3D models, which in many cases can be produced quickly with the printer, act here... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog PostPublishedFeb. 2, 2021, 2:15 p.m.... personal statement, while you make the ultimate case: why you? 7. Strength in numbers Encourage your ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....were conducted on how the results were analyzed. A casual reading of the study is not enough to uncover these... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog PostPublishedDec. 12, 2019, 2:22 p.m....strategies we used in our software design. In each case, we leveraged the team’s knowledge of the scientific... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m.... here. Don’t only talk about science and work – casual socializing is how people build relationships that... -
3 Challenges in Plant Synthetic Biology
TypeBlog PostPublishedJuly 22, 2014, 5:46 p.m....open innovation in plant synthetic biology. In our case, we selected a project in a field with 30 years ... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog PostPublishedJuly 10, 2014, 7:17 p.m....healthcare and the community. As a result, I have occasionally pondered translating my research into a startup... -
Plasmids 101: Yeast Vectors
TypeBlog PostPublishedFeb. 25, 2014, 7:11 p.m....markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School researcher...