Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 715 results for: lic

Showing: 501 - 520 of 715 results

  1. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... scientist position at Addgene, the skeptical applicant asked if she would partake in “critical thinking...

  2. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ... vehicle for making research data available for public access, review, and query. Gates Open Research ... both accept deposit of data associated with publications into one of five general purpose open source...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...Freedom: The Expansion of Data Sharing in Research Publications Resources at Addgene.org Check out our Protocol...

  4. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... the bacteria were injected, limiting their applicability. They also did not scatter ultrasound nonlinearly...promoter pBAD and a low-copy number origin of replication (p15A) worked best under a wide range of inducer...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...priorities in most cases include IP protection, publications and funding. If any of these three pillars crashes...working for a large institution or company, site licenses might be available as well. In most cases this...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ... progress. For this, I always make sure I have explicit goals in mind for the endpoints of a task or project...instance, if I don’t have draft blog post ready for publication for tomorrow, I’d better upload that post from...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... most start-up P.I.’s. It doesn’t have to be complicated, or difficult to create this list. Start BEFORE...discounts, collaborations, access to internal award applications, etc. If this is the institute that can help...

  12. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...that carried out the labor-intensive process of replicating your new plasmid: the Escherichia coli bacteria... Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! Mammalian Vectors...

  13. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... DNA from host cell nucleases (4). To further complicate matters, even when sequenced, phage genomes tend...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...

  15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...previously made mouse model. To avoid disrupting splice donor/acceptor sites, Miura et al. (2018) recommend...Used with permission under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR...

  16. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...et al (2). In this post, I’ll cover two great applications of the NLuc technology. The first couples NLuc...fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including monitoring...

  17. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...recession. As each advertised job received many applications, employers were requesting that only scientists...from other individuals projects, this can cause conflicts and favoritism, as well as affect morale and cause...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...but, in numerous studies, optogenetics is used in slice physiology in order to directly show that, for example...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...

  19. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...and gRNAs can be inserted using Gibson Assembly or SLIC cloning methods. A BsaI-based E. coli multiplexing... gRNAs can be assembled using Gibson Assembly or SLIC cloning. gRNAs are expressed from two Drosophila...

  20. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...original publication and ensure that it includes validation data relevant to your application — and double-check...
Showing: 501 - 520 of 715 results