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We narrowed to 821 results for: nin

Showing: 501 - 520 of 821 results

  1. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...depend on what downstream applications you are planning, cost, and the amount of protein you need to produce...popular choice for this. Chromatography columns containing appropriate immobilized ligands are incubated...incubated with cell culture supernatant containing antibodies. The antibodies bind the immobilized ligands and...

  2. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...retroviruses are RNA-containing viruses  (Robinson et al., 1965)! You may be beginning to realize that the...Lander, M. R., Pugh, W. E., & Teich, N. (1971). Noninfectious ARK mouse embryo cell lines in which each cell...

  3. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...continue such discussions. Beyond the excitement of learning about new cutting edge science, researchers of...the most important is letting people know it’s happening. Aim for 3-4 weeks advance notice for the first...presenter, figure by figure) if the primary purpose is training graduate students in critical reading. If the ...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...post, most bacteria have poor non-homologous end joining machinery, so a CRISPR-induced double-stranded ...phagemid delivery systems. Phagemids are plasmids containing a phage origin of replication that can be packaged...using phage-derived vectors to carry CRISPR, we’re turning the tables on the bacteria. Future research will...

  5. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...control elements for each would be relatively large. Cloning the viral genes in this plasmid would be more difficult...is important that all of these plasmids (those containing NP, VP35, VP30, L, and the minigenome) are efficiently...ways: transfecting with an additional plasmid containing the T7 RNA polymerase gene or using cells that...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...barcodes can be purchased from many companies such as Corning. Tubes can even have specific barcodes etched or...barcode reader and an Excel spreadsheet. Start by scanning the sample barcodes into the cells in the same...corresponding Excel sheet.3. Be specific  - When assigning a physical location to your samples, include as...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...Victoria and expressed in any organism while still maintaining fluorescence. The protein structure, first reported...reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore concealed at the...molecular biology. Scientists can easily utilize GFP-containing plasmids as a means to many functional ends. ...

  8. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...Addgene transfer plasmids are replication-deficient meaning they can be used to create virus that is capable...HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral transduction Q8: You mentioned...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...

  9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    Published
    Sept. 9, 2025, 1:15 p.m.
    ...sequences were then cloned into an AAV backbone containing a minimal promoter to drive expression of a fluorescent...guide to a smaller set of tried-and-true winners, containing enhancers with the highest specificity and brightness...just that, and their painstaking efforts towards screening thousands of enhancer sequences has culminated...

  10. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...structure is also reminiscent of sgRNAs, with reRNA containing two parts: a structural scaffold derived from... gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors... using your method of choice. Step 5: Colony Screening Use Primer 92F (5′-cattacgcaattggacgacaac-3′)...

  11. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...transient transfection, the vector remains episomal, meaning it stays separate from the genome. One option for...replicate during the course of your experiment, meaning you don’t have to worry about vector replication...example, if you are using E. coli simply for easy cloning and quick DNA production, you probably don’t need...

  12. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...their lab, in their experiments, to enable virtual learning during the pandemic. Feedback like that lets us... easier access to export-controlled items by streamlining permissions and paperwork. Through updating ...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

  13. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific... revealing antigenic differences between human, canine and simian isolates. J Gen Virol., 68 (Pt 11), ...transformed with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection...

  14. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ... Flow data is messy, so learning how to gate takes a lot longer than learning how to read a gating strategy...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...

  15. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein into the lentiviral envelope...Table 1 (Gutierrez-Guerrero et al., 2020) Table 1 (Cronin et al., 2005)     References and Resources References...Viruses 12:513 . https://doi.org/10.3390/v12050513 Cronin J, Zhang X-Y, Reiser J (2005) Altering the Tropism...

  16. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    Published
    April 13, 2021, 1:15 p.m.
    ...this point, subsequent introductions of pTargetF containing another sgRNA targeting another site of interest...specific sequences on pTargetF with the ccdB gene containing BsaI restriction enzyme sites at its ends to ...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

  17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...determination, and anterior-posterior segmentation (Jennings and Ish-Horowicz, 2008). Fruit flies that contain...when she pricked her finger on the spindle of a spinning wheel but also a biological transposon system....: 21314430. PubMed Central PMCID: PMC3130349. Jennings, Barbara H., and David Ish-Horowicz. "The Groucho...

  18. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...eventually called aptamers, from the Latin aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers... and tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Chemistry 27(2):108-117 · February 2008. 11. Svensen, Nina and Jaffrey, Samie R. “Fluorescent RNA Aptamers ...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...antibodies. The most famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc),...Looger’s lab have developed new molecular tags combining the advantages of both (Viswanathan, 2015). For... in better resolution images. Conclusion By combining advantages of FPs and epitope tags, smFPs appear...

  20. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...to the AMA, we had discussed the possibility of joining the conversation on Reddit, but it wasn’t until... pleased to see graduate and postdoc programs beginning to support diverse career path development--but...resources. The internet content for scientists in training is abundant, helpful, and sometimes excellent....
Showing: 501 - 520 of 821 results