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TypeBlog PostPublishedMay 11, 2017, 2:30 p.m.... This post was contributed by Jesse S. Boehm, the Associate Director of the Cancer Program at the Broad...
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Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... sign up here. Here's what you'll find in this post: Anti-FLAG Frankenbodies for modular imaging of... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....If you’ve been following our antibody blog posts, then you likely already have a good idea of the basics... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....can use antibodies for in our Intro to Antibodies post Check out our Antibodies 101: What is an Antibody... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....This post was contributed by guest blogger, Eugenia Rojas. A question worthy of a PhD: How do you visualize... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m.... resources on the Addgene blog Browse our blog posts on base editing Read more about CRISPR Learn all... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m.... on the Addgene Blog Read the Plasmid 101 blog posts Check out CRISPR Meets Synthetic Biology: A Conversation... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m.... sign up here. Here's what you'll find in this post: Improving flexibility for in vivo proximity labeling... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog PostPublishedJuly 21, 2016, 2:30 p.m....The post was contributed by guest blogger Londa Schiebinger, PhD, Hinds Professor of History of Science... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog PostPublishedJune 2, 2016, 2:30 p.m....This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m.... within this range. Read some of our other blog posts for more tips on sequence analysis, plasmid verification... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m.... protein tagging with CRISPR Additional CRISPR Posts from the Addgene Blog ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....summarized the three strategies described in this post in the graphic below - including some simple comparisons... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....In previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....This post was contributed by Neil J. McKenna and Bert W. O’Malley. Survival of all organisms depends ... -
Delivery Methods for Generating iPSCs
TypeBlog PostPublishedApril 17, 2018, 1:37 p.m....reprogrammer, to decide which method to use. This post provides a brief overview of reprogramming methods...reprogramming factors. Check out the links at the end of the post for a list of Tet-inducible reprogramming vectors... -
CRISPR/Cas9 FAQs Answered!
TypeBlog PostPublishedMarch 13, 2014, 4:08 p.m.... As Kendall mentioned in Tuesday's blog post, keeping up with the newest CRISPR technologies and their...resources with Addgene. Thank you, Le! This blog post was updated to include all FAQs from Le Cong on ... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....on how to use systemic capsids, when writing this post, we didn’t come across a broadly used working definition...ability to cross barriers like the BBB.) In this post, we’ll discuss how systemic capsids are engineered...of options for systemic capsids, but we hope this post helps you find the right one to test in your application...finished!) Good luck and happy transducing! This post was written by Rachel Leeson, with significant contributions... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....have used relevant viruses for your system. This post was contributed by guest blogger Didem Goz Ayturk...Guerin and Susanna Stroik. Didem Goz Ayturk is a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal...