We narrowed to 521 results for: she

1. Fluorescent Proteins 101: When GFP lets you down

   Type

   Blog Post

   Published

   Aug. 23, 2018, 12:05 p.m.

   ...1731-1745. PubMed PMID: 26960991. 9. Chatterjee, Abhishek, et al. "A genetically encoded fluorescent probe...

2. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   Blog Post

   Published

   March 1, 2016, 3:30 p.m.

   ... GeneArt® Seamless Cloning and Assembly (Thermo Fisher Scientific) NEBuilder® HiFi DNA Assembly (NEB) ...

3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   Published

   April 5, 2018, 12:42 p.m.

   ...PAM (bold highlighted text) and cleavage sites (slashes) are shown. In this case, the green guide would...

4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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   Blog Post

   Published

   Jan. 16, 2025, 3:42 p.m.

   ...unlock” and infect specific cells. This protein shell packages the vector’s genetic material, and variations...

5. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

6. Antibodies 101: Multiplex Immunofluorescence

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   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ...the secondary antibody, it should get completely washed away and leave no signal. If you see lots of extra...

7. Generating Mouse Models Using CRISPR/Cas9

   Type

   Blog Post

   Published

   July 12, 2016, 2:30 p.m.

   ...world, I (Wenning) was fascinated by CRISPR and rushed to test it. I still remember the day, March 16,...

8. Plasmids 101: Restriction Cloning

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   Blog Post

   Published

   Feb. 18, 2016, 3:42 p.m.

   ...ratio” in the ligation reaction. 5. Purify the finished plasmid   Once it looks like your ligation has...

9. Quick Guide to All Things Lentivirus

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   Blog Post

   Published

   March 21, 2017, 2:30 p.m.

   ... generations of lentiviral vectors have been established so far, with safety increasing in each generation...

10. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    Published

    Oct. 27, 2020, 1:15 p.m.

    ...837597 Li J-F, Chung HS, Niu Y, Bush J, McCormack M, Sheen J (2013) Comprehensive Protein-Based Artificial ...

12. Custom CRISPR Screens & the Green Listed Software

    Type

    Blog Post

    Published

    July 11, 2017, 2:30 p.m.

    ...like to use in your screen. Currently, eleven published libraries are embedded. These were kindly donated...

13. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ... tagging is scalable to 96-well format. Off-the-shelf CRISPR reagents for the knock-in experiment were...

14. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... Alyssa Shepard...

15. Antibodies 101: Flow Cytometry

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    Blog Post

    Published

    July 20, 2021, 1:15 p.m.

    ...first resuspended in a pressurized buffer called sheath fluid and transported through tubes or capillaries...

16. Antibodies 101: Introduction to Antibodies

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    Blog Post

    Published

    Jan. 19, 2021, 2:15 p.m.

    ...out of the solution. Once the unbound protein is washed away, the protein of interest is dissociated from...

17. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    Published

    Sept. 10, 2015, 2:30 p.m.

    ...the many primary research papers that have been published at this point.  In brief, some standard opsins...

18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...

19. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...Endotoxin-free, transfection-grade, BAC Thermo Fisher Beads, column, plate Mini, midi, maxi, mega, giga...

20. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ...analyzing qPCR data, most with plug-and-play Excel spreadsheets available for easy analysis. These useful tools...