We narrowed to 740 results for: NSI

...system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two components...scFv-GFP fusion bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables...to the disulfide bond independent folding of intrinsically stable scFv. PLoS ONE 12:e0189964 . https://...which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on Addgene.org...

...have, and we developed a great plan to start consistently knocking out high-quality videos for you all...two options: Film outside at a distance, or film inside with one person in a room. I wouldn’t be in there...live. After doing a bit of looking, I found an inexpensive HDMI transmitter that could transmit a signal... stand and mounted a monitor to it. It had an extension cord attached, with the option to mount a battery...

...called a katana. Opto-katanin consists of two separate blue light-sensitive systems, VVDfast to control ...switch. You may be familiar with popular light-sensitive ion channels used to control activities like neuronal...in other contexts are sure to reveal even more insights. Super-duper lipid editor And if recruiting proteins...

...mechanisms, or ascertain which integrins are responsible for specific ligand-binding functions, all key...microenvironment. The actin cytoskeleton exerts tensile force, transmitted through the integrin, where ...recently showed RGD-binding integrins demanded higher tensions before they would enable cells to spread, resulting...symmetrical circles. While elucidating the role of tension, however, the researchers realized they needed ...

... clients focus on their experimental needs and consider a budget as a resource checklist. A budget is ...centrifuges, sequencers and analyzers, and any ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes...awry when several factors aren’t taken into consideration. 1. Always focus on the science When negotiating...However extreme a solution may seem, you should consider it if it will help you achieve your research goals...

...the results across runs. If the results vary considerably, then the high degree of variability in the ...assays have their own unique controls that must be considered (Burry 2011). Perhaps the most critical are those...materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit less ideal...signal remaining is autofluorescence and should be considered in the interpretation of results. Include the...

...first.  AAV-mediated gene expression can differ extensively depending on the experimental system used, so...Watakabe et al., 2015) (Figure 1). You’ll need to consider if such properties could help, or harm, your experimental...occur (Fig. 3) - for example, some neurons can transiently express TH - so you’ll need to check for this...injection volume.  There are a few factors to consider. First, the titer required is inversely related...

... have engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1 promoter...systems. Binary systems are genetic tools that consist of two parts, both of which must be present for...results in chimeric GAL80-repressible and GAL80-insensitive activators of lexAop. This system is commonly...expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci U S A. 1992 Jun...

... sequencing would be too expensive, then the qPCR method should be considered as an alternative. The qPCR...three-input GRSMs, we recommend using overlap extension PCR and Gibson assembly to piece together the ...circuits in general would benefit from a more comprehensive characterization of the recombinases at our ...

...encode a transposase enzyme.  Class I TEs are considered replicative since every time they jump they make...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Like Sleeping Beauty, piggyBac has ...transcription start sites, CpG islands, and DNaseI hypersensitivity sites. Tol2 is only active in vertebrates ...site preference and host range are important to consider when selecting a transposon for a mutagenesis ...