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Showing: 781 - 810 of 885 results
  1. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
  2. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...
  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...
  4. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...
  5. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...for your audience. I ask myself that question constantly (and so should you!)  Amount of information and... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...
  6. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...
  7. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...
  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus...
  9. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are...
  10. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...and the distances the packages needed to travel meant the accessibility varied country to country.   Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...
  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and... (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of...
  12. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations...
  13. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...don't have to take our word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia...
  14. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...
  15. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...all published videos in our Quick Tips playlist:  Want your quick tips even quicker? We’ll be sharing our...
  16. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...sweaters, Flannel Fridays, Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout...
  17. CRISPR Guide Update

    Type
    Blog Post
    Published
    Oct. 10, 2024, 1:15 p.m.
    ...CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions describing...
  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...families, eating delicious food, or playing one of the giant games or indoor mini-golf course.    While the main...
  19. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications...
  20. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...Thus, we are in a unique position to observe and quantify how new technologies are being disseminated through...
  21. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author...
  22. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...fact, our Addgenies were absolutely abuzz with anticipation as they cast their ballots for their favorite...
  23. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ...On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene...
Showing: 781 - 810 of 885 results