We narrowed to 885 results for: ANT;
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TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...
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Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....for your audience. I ask myself that question constantly (and so should you!) Amount of information and... too little)? The answer is: it depends! The important point here is to pay attention to exactly what... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....Modifying gene expression Generally speaking, one may want to achieve either inhibition or activation of neurons...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion. In addition to the obvious advantages SapTrap offers for tagging a single genetic locus... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....and the distances the packages needed to travel meant the accessibility varied country to country. Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene. In our next... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and... (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m.... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....don't have to take our word for it! “When people want to learn about plasmids, they don’t go to Wikipedia... -
Beginner's Guide to Viral Vectors
TypeBlog PostPublishedJune 2, 2017, 2:30 p.m....Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,... -
Introducing...Addgene Quick Tips!
TypeBlog PostPublishedSept. 19, 2024, 3 p.m....all published videos in our Quick Tips playlist: Want your quick tips even quicker? We’ll be sharing our... -
#DeckTheLab to Win a Tasty Winter Treat This Holiday Season
TypeBlog PostPublishedNov. 30, 2015, 3:30 p.m....sweaters, Flannel Fridays, Festivus— you name it, we want to see it. All photos tagged with #DeckTheLab... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog PostPublishedSept. 7, 2021, 1:15 p.m.... courses. The Intro to the Lab Bench series is meant to be an informative introduction for those who ... -
'Tis the Season to #DeckTheLab
TypeBlog PostPublishedDec. 2, 2016, 3:30 p.m....sweaters, Flannel Fridays, Festivus— you name it, we want to see it! All photos tagged with #DeckTheLab throughout... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....CRISPR Guide here! Highlights include a much-anticipated prime editing section and additions describing... -
Suggest A Plasmid You'd Like to Find at Addgene
TypeBlog PostPublishedMarch 16, 2016, 5:30 p.m....where we can find the plasmid. While we cannot guarantee that everyone we reach out to will end up depositing... -
Addgene's 20th Anniversary Party
TypeBlog PostPublishedJune 11, 2024, 1:15 p.m....families, eating delicious food, or playing one of the giant games or indoor mini-golf course. While the main... -
It’s CRISPR Week at Addgene!
TypeBlog PostPublishedMay 13, 2019, 12:01 p.m.... CRISPR and Wednesday will highlight some Cas9 variants. Next, we’ll look at various CRISPR applications... -
Trends in CRISPR and SynBio Technologies
TypeBlog PostPublishedFeb. 4, 2015, 3:58 p.m....Thus, we are in a unique position to observe and quantify how new technologies are being disseminated through... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog PostPublishedDec. 5, 2023, 2:15 p.m....Codon Guide to Synthetic BiologyAuthor: Niko McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog PostPublishedApril 1, 2021, 1:15 p.m....and enter the raffle. We encourage you to take advantage of the blog’s newly updated navigation menu and... -
Overwhelmed? Take a Break With Our 5 Favorite Science Comics
TypeBlog PostPublishedDec. 5, 2013, 3:27 p.m....Researchers in Taiwan showed that a laughter program significantly decreased immunological markers of stress in... -
2021 Deck the Lab Winners!
TypeBlog PostPublishedJan. 25, 2022, 2:15 p.m....fact, our Addgenies were absolutely abuzz with anticipation as they cast their ballots for their favorite... -
The Twelve Days of CRISPR
TypeBlog PostPublishedDec. 22, 2020, 2:15 p.m....On the eighth day of CRISPR, Addgene gave to me: anti-CRISPR proteins! On the ninth day of CRISPR, Addgene... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog PostPublishedSept. 5, 2018, 12:01 p.m.... the folks behind the journal and hear from a fantastic team of high school students who recently submitted...