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We narrowed to 582 results for: BASE;

Showing: 521 - 540 of 582 results
  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...
  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The VSV-G envelope...
  3. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...
  4. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...
  5. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...Think a university system who develops evidence-based university policy to combat sexual harassment in...
  6. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...
  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...
  8. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...
  9. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...
  10. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...
  11. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...
Showing: 521 - 540 of 582 results