We narrowed to 583 results for: BASE;

...reporters have limited use in vivo because they are based on light which is very easily scattered by tissue...

...genomic edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to ...from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it can be packaged into...

...discipline are welcome. Up to this point most videos are based on research that has been published, but many discuss...

...representing ~85% of the postdoctoral workforce. Based on our communications with and research on these...

...should first develop primers to the approximately 500 base pair sequence surrounding your gRNA. Once amplified...

...could reveal something fascinating. Behavior is based primarily on careful observation of what an animal...

... smaller, with the full sequence being around 60 base pairs. This is much shorter than miRNA plasmids,...

... the meaning of the word ‘fact’. Policy must be based on our most accurate interpretation of the world...

...fail to recapitulate the true steric and charge-based nature of the phosphoryl-modification; they are ...appreciated. PhosphoSitePlus, one of the most inclusive databases of post-translational modifications, identifies...

...same conditions. Example: You want to sort cells based on the expression of a new receptor that is of interest...attempts by other means, such as through antibody databases like Antibodypedia or through your antibody provider...

...Think a university system who develops evidence-based university policy to combat sexual harassment in...

...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519.e17. ...

...WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations...

...interest.  Determine injection titers and volumes based on the serotype Since each serotype also exhibits...

...electrical activity to gene expression, and even CRISPR based gene editing. But, how do you decide which tools...

...deficiency independently increase the frequency of base substitution in human cells. J Biol Chem. 2011 Mar...

...Addgene’s main offices are in Cambridge MA, while I’m based in London) does bring its own challenges. I’ve had...

.... Eggeling C, Willig KI, Sahl SJ, Hell SW. Lens-based fluorescence nanoscopy. Quarterly reviews of biophysics...

...PMC4772972. 11. Salomonnson, Emma, et al. "Cell-based and in vivo spectral analysis of fluorescent proteins...

...chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila... recovery of knock-ins, but did result in 1-2 kilobases of additional sequence being introduced. Cre-mediated...repair templates (ie. PCR-derived dsDNA) with 30-60 basepair homology arms to knock in large epitopes, such...