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We narrowed to 902 results for: Ide

Showing: 521 - 540 of 902 results

  1. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...certainly hope not! One of our core values here is to provide unmatched customer support, as this is vital to...exact problem, and it’s called “self-service.” The idea here is to build features that allow users to help...general question or concern about the answers we provide, within the widget you also have the option to ... we’ll be continually improving the support we provide based on your valuable feedback. This is Addgene...first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features on Our Plasmid...

  2. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014 Johnson & Johnson’...up to the discovery of the mechanism of dual-RNA-guided DNA cleavage in adaptive bacterial immunity and...Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity” driven...thanked Martin Jinek and Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their... Technology? Learn More: Addgene's CRISPR/Cas9 Guide History of CRISPR Cas - A tale of survival and evolution...

  3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ... mapping where a protein binds genome-wide, and has been widely applied in many model organisms and cell... Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that overcomes some of the... degree of signal to noise is desired. It also provides an excellent additional measure of DNA binding...technique will quickly become a standard method alongside ChIP. Many thanks to our guest blogger, Matthew...

  4. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...clinical data about a disease or therapeutic Side-by-side comparison of the same experiment from different...makes your insights discoverable. Watch this quick video to learn how ReFigure works and continue reading...Apps to Save Time and Track Your Experiments Use video to share your science at We Share Science Learn ...with Addgene Find Molecular Biology Protocols and Videos Find Other Molecular Biology Resources ...

  5. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors When choosing a biosensor, you first need to decide the best way to track or measure the process you... of small molecules like arabinose or hydrogen peroxide or ions like calcium and zinc. Some biosensors...compatible with your lab and experimental setup? Consider what kind of instrumentation you have available...components of individual cells Separation/identification of fluorescently-labeled components Fluorescence-activated...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Visit our viral vectors guide Find our chemogenetics and optogenetics science guides ...Chemogenetic approaches excel at broad tissue or system-wide targeting, as the ligand can reach target cells ...optogenetics. If broad control of whole circuits or tissue-wide systems is necessary, use chemogenetics. Note that...approaches suffer from unique sets of off-target side-effects that should be carefully controlled for ...

  7. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    Published
    Oct. 18, 2017, 1 p.m.
    ...post-grad plans, but in the end, writing won out. “I considered teaching at a liberal arts school but I’ve always...Iowa, but kept up a freelance writing hustle on the side. “I actually stumbled upon, about 6 months in, this... was for Cactus Communications, a company that provides scientific editing services. “I was working during...At the end of her postdoc, Caitlin had a clearer idea of her career goals: “My plan was to go into editing...landing her job. All of the hard work she’d put in outside the lab had paid off. “The combination of the editing...

  8. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ...service that we, as scientists, provide for journal editors to help decide whether work is suitable for ...of the 18th century. Yet the peer-review was not widely adopted by the scientific community until the mid...peer-reviewing journal submissions since 2015 for a wide variety of journals. Since then, I developed a routine...submitted paper in addition to the journal-specific guidelines.  Peer-review step 1: The first read At the first...

  9. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ..., Addgene will have unique Research Resource Identifiers (RRIDs) for each antibody, making it easy for...topics are discussed on Addgene’s blog and Antibody Guide, which include information about immunofluorescence...suggestions about how to make the resource better or ideas for new materials to add to the collection, please...find useful antibodies for your research. Please consider sharing your experiences with antibodies from ...Introduction to Immunofluorescence Addgene’s Antibody Guide The project is supported by a NIH BRAIN Initiative...

  10. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...transformation fall into two main categories: antibiotic/herbicide resistance and fluorescent proteins. Selection...undifferentiated plant cell culture), which is great for a wide variety of applications. But some techniques require...CRISPR-Cas9 gene editing, transformation may be identified by resistance to an antibiotic. Successful editing...therefore, are useful for research, and they are identified by a phenotype caused by the segregating mutation...these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my work and ...

  11. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type
    Blog Post
    Published
    April 12, 2018, 1:02 p.m.
    ...developed and tested by ABRC staff. These kits provide in-depth, ready-to-teach lab protocols and supporting...supporting materials such as instructional videos and datasheets. The remaining kits were donated to ABRC...cycle, and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science...outlined in ABRC’s kits vary by module. As a general guideline, experiments that include growing plants require...available via e-mail and phone to answer questions and provide support. Growing capacity - ABRC outreach today...

  12. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type
    Blog Post
    Published
    March 9, 2017, 3:40 p.m.
    ...demonstrate that 85% of leaving postdocs pursue a career outside the traditional academic silo to tenured professor....  Here are some questions and perspectives to consider. What do academic and non-academic careers in ... a greater sense of autonomy - there are fewer outside influences on your topics of research or course...move around every 3-6 years—a short and exciting ride Complicated matrix structures are common Small... a scientist you enjoy the most. Having a better idea of where you want to go will help you focus on the...

  13. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...useful, chemical fluorophores can still be used in a wide variety of experiments. Read more to find chemical...potential applications of R-bodies and to find a video of them in action. AAV: A Versatile Viral Tool for...interest to a variety of cell types in-vivo. These are widely used by the optogenetics and chemogenetics communities...pathways using light and chemicals respectively. Didem Goz Aytruk from Connie Cepko’s Lab at Harvard Medical...AAVs function and indicates important things to consider if you’re getting started with them. CRISPR Pairing...

  14. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...resources and experience to their advantage should they decide to produce virus on their own.Harvard researcher... What viral preps are available now? There are a wide variety of tools already available from Addgene ...be continually expanding our collection. We’ll provide a brief rundown of what lentiviral and AAV preps...it, modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course...the Addgene website. blog.addgene.org The blog provides tips for working with viruses including advice...

  15. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...a new CRISPR screening method that makes use of guide RNAs with internal barcodes (iBARs) in their loop...experimental replicates for each of the gRNAs. The idea was to assign four different barcodes to each of...covers every annotated human gene, it enables genome-wide screening with a high MOI, which is beneficial for... and can serve as a drug-screening platform to identify new antifungals that target calcineurin. Current...

  16. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...important to use a high fidelity polymerase to minimize mutations. The fidelity of the polymerase becomes...as Addgene’s Sequence Analyzer, to allow you to identify which restriction sites are present in a given...compatible with a standard PCR reaction (see PCR Video). Therefore, our Forward Primer will use the sequence... chance for mis-priming, so you can use a pretty wide range of annealing temperatures, but you may need...

  17. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...this stage, only cells from the immune system show wide scale mobility with movement from the bone marrow...you can detect signal in thick specimens. See the video below for an example of two-photon intravital microscopy... in diverse tissue environments. These studies provide quantitative measurements of cellular motility,...and skin. Two-photon intravital microscopy. The video above depicts the infiltration of neutrophils expressing...technical specificities have to be taken into consideration especially in the use of fluorescent proteins... Addgene.org Check out Our Fluorescent Protein Guide Pages Find Plasmids for Subcellular Localization...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ... the widely used 293T. During vector production, assembled AAV vector particles accumulate inside the ...either associated with the outside surface of the exosome or are carried inside the vesicle, which may also...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

  19. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...situation. But a few days later we had an idea. And this idea rapidly grew and became Crowdfight COVID-...modern science in our view. And it was this very idea that research is done better with others than by...has been essential to push certain projects. The idea behind the platform was simple: any person working...remote collaborations Read our Transferable Skills Guide article about external collaborations Resources...

  20. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...working in academia or industry. At BosLab, we identify ourselves as part of a network of independent ...square, but we saw the potential in the space and decided to reactivate it. We started out by holding a few...from hacking probiotics to filming educational lab videos, while some are looking to develop new skill sets... mind. Thus, to encourage more involvement and provide all our members the opportunity to do molecular...project. This project was inspired by previous city-wide genomic studies and our study will involve getting...
Showing: 521 - 540 of 902 results