We narrowed to 791 results for: Kin
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TypeBlog PostPublishedApril 25, 2023, 1:15 p.m....You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype...
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What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m.... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you? If you are looking for a human cell line model, HEKs might be a great... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog PostPublishedMarch 28, 2022, 4:30 p.m....catalog, so that you will know exactly what you are working with. We will also be collecting feedback from ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ... -
RUBY-Red Siliques
TypeBlog PostPublishedMarch 1, 2022, 2:15 p.m.... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog PostPublishedAug. 11, 2020, 1:15 p.m....experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV: the... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m.... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m.... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog PostPublishedApril 25, 2019, 3:01 p.m....484 variants, including some potential tetramer-breaking mutations. The library was then screened through...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m....properties (for example, overall brightness or on/off kinetics) that makes them suitable for different applications...with a CAG promoter exhibits high specificity for Purkinje neurons. 105321 AAV1 pGP-AAV-syn-jGCaMP7c... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story. At this point you may be thinking “I don’t want to bury my results all the way ... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated... -
Trading Spaces - Visiting Scientist Edition
TypeBlog PostPublishedAug. 22, 2023, 1 p.m....you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist...really good time! If it sounds right for you, start thinking outside of your current research box, send out... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m..... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos. Tools for checking grammar and jargon Hemingway Editor This is a... -
New and Upcoming Viral Vectors - June 2019
TypeBlog PostPublishedJune 18, 2019, 1:15 p.m....contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....Incredibly imaginative, funny, bittersweet, and shocking at times." - Jason Snair, Product Designer...on today! Addgene Book Club (ABC) founder, Eric Perkins, is Addgene’s Director of Science Product Management...