Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 839 results for: LEA

Showing: 521 - 540 of 839 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...receptor-transcription factor fusion. TEV protease cleave the transcription factor from the receptor, which... in chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime...02775-8 Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics...

  2. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously mentioned...the recommender knows you the better, and their leadership status in their current field of expertise augments...But you create a network of friends, and future colleagues that can help pull one another up while you are...

  3. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    Published
    July 28, 2017, 1:30 p.m.
    ...This is just a primer so that you are aware of at least some of the possibilities. Basically, if you can...between pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince...PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read Other Genome ...

  4. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... Addgene’s high quality standards?” Under the leadership of Addgene’s co-founder, Melina Fan, we created...Production webpage). While the new team took the lead on operationalizing viral production and distribution...Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in Your Research...

  5. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...our Viruses as Biological Tools blog can help you learn about the different types of viruses and pick one..., McPhee DA, Verity E, Dyer WB, Wesselingh SL, Learmont J, Sullivan JS, Roche M, Zaunders JJ, Gabuzda ...Resources on Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral...

  6. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...’s wasting everybody’s time if you don’t. Add a clear cover letter to your submission, briefly summarizing...house style and prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread...that all required information is provided. The clearer you write your paper and the more information you...

  7. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode ...Acr activity as a biological tool, Niopek and colleagues turned to optogenetics. Through the use of light-sensitive...usually expressed from a different plasmid, which may lead to unequal gene dosage and high variability of protein...

  9. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...proteins in situ. I was privileged to be invited to learn from them at the MPI in Frankfurt am Main and perform...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...Fluorescent microscopy techniques - Which is best for me? Learn how small molecule fluorophores can be used for ...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...targets. Check out our post on Biotinylation to learn more about biotin and its use in molecular biology...other classes that we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection... with BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ... works best for genome editing  with an active nuclease.  Read the paper in Molecular Cell Find the CasMINI...Programmed genome editing by a miniature CRISPR-Cas12f nuclease Quanjiang Ji’s lab describes an effective miniature...protect the RNA from degradation by cellular exonucleases and broadly improve editing efficiency. Read...

  12. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...people said it took them more than 22 hours, so at least the better part of four days. How much does it cost... a number of factors, but we can assume it’s at least equal to the $155 spent on reagents. Five ways to...tell. But even from these rough estimates, it’s clear that community engagement with and use of repositories...

  13. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...within the resistant populations were shared by at least one other replicate; prior to treatment, there are...particularly interested in applying what we have learned about tumor heterogeneity to drug discovery and...PMC4900892. Additional Resources on the Addgene Blog Learn about the Sabatini and Wood Drug Screening Kit Find...

  14. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ... papers is to practice reading them. There’s a learning curve for the jargon and background in your field...the reader to interpret the results to see if it leads you to the same conclusion. Personally, I like papers...interest. Set aside some time each day or week to at least scan the titles of what’s going on in your research...

  15. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...analyzes publications and figures using machine learning to extract important features such as influential...you organize and search for papers later. Pro tip Learn more about authors by exploring author pages (for...literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter Read our Early...

  16. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...Washington DC; convening meetings of experts and leaders from academia, industry, community, education and...the biological sciences, as well as to acquire leadership and communication experience. These included ... science career path Resources on Addgene.org Learn how Addgene engages with the community on our Addgene...

  17. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references...purified plasmid stock should be analyzed by endonuclease restriction digest or fully sequenced to confirm...a week), and do NOT harvest the cells before at least 10 days of incubation as it will result in very ...

  18. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV Quality Control...Self-complementary AAV mediates gene targeting and enhances endonuclease delivery for double-strand break repair." Gene...

  19. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...Genes allowing bacteria to survive toxins are the clearest example of a plasmid benefiting its host. For ...engineered entirely new types of plasmids without clear analogues in nature. For example, helper plasmids...plasmid tools available in the Addgene repository. To learn more about some other common plasmid features, such...

  20. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...reprobing, maximize your signal by starting with the least-abundant protein and working toward the most abundant...primary and secondary antibodies from your membrane, clearing the way for the second blot. There are multiple...with the mild one. Reprobe Once your membrane is clean of antibody, you’re ready to repeat your western...
Showing: 521 - 540 of 839 results