We narrowed to 711 results for: Met
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TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....speaking, the PhD application process tends to go something like this: However, as simply as it seems laid...program. An email to a potential PI should go something like this: Introduce yourself, brief educational...
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Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m.... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...the circulatory or lymph systems to spread, or metastasize. Many cancerous cells will die trying, as the... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....it at the time, but gene therapy had fallen - plummeted really - from grace 2 years prior with the death...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m.... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...relationship is non-linear and you should use a different method of analysis. Different fields and assays have varying... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m....step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to fuse an ...jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....barcoded tube (see next section) and every time something is removed from this tube, the barcode on the ...digit barcode and then assigned to a location in a metal freezer rack that contains five levels that can ... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m....Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... populations in drug screens, to visualize micrometastases in nude mice in cancer studies, act as a reporter... -
To Codon Optimize or Not: That is the Question
TypeBlog PostPublishedNov. 12, 2020, 2:15 p.m....improves expression levels. Journal of Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(...base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna... -
Four Base Editing Reporters to Monitor and Enrich Editing in Real-time
TypeBlog PostPublishedJuly 7, 2020, 1:15 p.m....of base-edited cell populations. To develop this method the lab was inspired by work that used an integrated...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m....essential for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...with far fewer side effects compared to lesioning methods - they may be the key to further unraveling the... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many... editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory of ... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m....vectors. While viral delivery is a highly effective method for gene editing and allows targeting of specific...annealed oligos into the digested vector using your method of choice. Step 5: Colony Screening Use Primer... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....enzymes (BsaI, BbsI/BpiI, BsmBI), which recognize asymmetric DNA sequences and cleave at a short distance ...stocks beforehand. You can also use high-throughput methods to streamline your plasmid extractions. Use this... -
Hot Plasmids - November 2023
TypeBlog PostPublishedNov. 14, 2023, 3:35 a.m...., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels... of proximity labeling in living cells. Nature Methods, 20(6), 908–917. doi: 10.1038/s41592-023-01880-... -
Transferable Skills Guide: Career Planning Resources
TypeBlog PostPublishedNov. 14, 2017, 2 p.m....Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....companies have dedicated technical support teams, sometimes comprised of several layers of support: A front-line...listen to the many individual stories and get (sometimes refreshing) insights into how other scientists... -
Using Video to Share Your Science: We Share Science
TypeBlog PostPublishedNov. 3, 2016, 2:30 p.m...., or pretty much anyone applying the scientific method to a question. Videos from any discipline are welcome...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...