We narrowed to 716 results for: Met

...from contaminants using a variety of purification methods. Deciding which expression system to use depends...transiently or through stable cell lines. Both methods produce high protein yields if transfection is ...

...little range for detectable improvements in the methodology. To further push the test system, we redesigned...fragments). The future of assembly DNA assembly methods are important tools for many areas of science, ...

...down the question you were trying to answer, the method you used, the main result, and the fundamental ...you’ve already set yourself up for success. But sometimes, attendees can get sucked in to thirty-minute-...

...degron tags can offer over other protein control methods and compare the commonly available types of degron...protein post-translationally, unlike many other methods. Secondly, these tags can be used to manipulate...

...these electrical events! Voltage indicators are voltmeters for cells! In this blog we will review how these... described systems. Several adaptations of this method have been to tag, cage, uncage, and trap the chemical...

... in place to protect us from rogue cells when something does go awry. The process of programmed cell death...the circulatory or lymph systems to spread, or metastasize. Many cancerous cells will die trying, as the...

...developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses ” (Cullen...viruses and transduction by vectors (which we sometimes still call “infection” as a shorthand, although...

...many facts but the training of the mind to think something that cannot be learned from textbooks” (Brian,...are often associated with rote memorization of metabolic pathways (precisely the type of exercise Einstein...

...step (Moreno-Mateos, et al., Nat Com). Another method used to prime a Cas break for HR is to fuse an ...jbc.TM118.000372 Yang, H., Ren, S., Yu, S., Pan, H., et al. Methods favoring homology-directed repair choice in response...

...journal club. This can be a challengingly vague metric but there are a few things you can point to. Does...receive include the sense that people learned something new? Did the meeting begin and end on time? Did...

...it at the time, but gene therapy had fallen - plummeted really -  from grace 2 years prior with the death...muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway and reported results...

... absorbance of both is then measured on a spectrophotometer and concentration versus absorbance is plotted...relationship is non-linear and you should use a different method of analysis. Different fields and assays have varying...

...barcoded tube (see next section) and every time something is removed from this tube, the barcode on the ...digit barcode and then assigned to a location in a metal freezer rack that contains five levels that can ...

...Fluorescence-activated cell sorting (FACS): This is a type of flow cytometry that separates mixtures of cells into distinct... populations in drug screens, to visualize micrometastases in nude mice in cancer studies, act as a reporter...

...Biospace holds career events all over the country; sometimes several per month, giving you a plethora of opportunities... Post your CV and make it visible, which will sometimes result in a recruiter giving you a call. Many ...

...companies have dedicated technical support teams, sometimes comprised of several layers of support: A front-line...listen to the many individual stories and get (sometimes refreshing) insights into how other scientists...

...of base-edited cell populations. To develop this method the lab was inspired by work that used an integrated...fluorescence that can be visualized and sorted via flow cytometry. Figure 3: Targeting pEF-BFP with a cytidine...

...essential for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...with far fewer side effects compared to lesioning methods -  they may be the key to further unraveling the...

...improves expression levels. Journal of Neuroscience Methods 124:75–81 . https://doi.org/10.1016/s0165-0270(...base-pairing slows in vivo translation elongation in metazoans. RNA 17:2063–2073 . https://doi.org/10.1261/rna...

...editors, keep in mind that the generation of indel sometimes may also results in RE site destruction. Many... editing in rice and uses base editors and HDR methods for rice crop improvement in the laboratory of ...