We narrowed to 850 results for: NTS
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TypeBlog PostPublishedOct. 10, 2017, 1:57 p.m....capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent widefield microscope...your institution when heading into a new set of experiments. Many thanks to our guest blogger, Doug Richardson...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent...
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Hot Plasmids: Bacillus subtilis Libraries, iPSC Reprogramming, CRISPR Tools, & More!
TypeBlog PostPublishedDec. 11, 2017, 2:02 p.m....CRISPR tools for use in plants. Listen to the segment on CRISPR tools for plants. Special thanks to our...enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly... -
Addgene is Stopping Use of X (formerly Twitter)
TypeBlog PostPublishedJan. 11, 2024, 2:15 p.m....scientific communities and welcome suggestions in the comments. X has moved significantly away from the functionality... Every community benefits from a diversity of viewpoints, and we acknowledge there may be a variety of...invite you to follow us across our social media accounts. -Addgene ... -
New Videos: Addgene Lab Tips
TypeBlog PostPublishedFeb. 15, 2018, 2:41 p.m.... Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them thoroughly...before use. For efficient mixing, add the various reagents to a single tube and simply flick the tube several...everything Use barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog PostPublishedMarch 25, 2025, 1:15 p.m....removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil ...developed two fusions of a CBE with other BER components: the DNA repair protein rXRCC1, or the DNA binding...both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE and cOPTI-CGBE had...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs... -
5 Science Rap Videos & Their Creator: Tom McFadden
TypeBlog PostPublishedFeb. 9, 2017, 3:30 p.m....Tom McFadden, recently worked with high school students in the bay area to create a plasmid rap video ...has made many more Science rap videos to teach students around the globe and is pushing SciComm further...DNA. 2. The Immortal Rap of Henrietta Lacks Students in Oakland rap about Henrietta Lacks - the African... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog PostPublishedJan. 23, 2018, 2:04 p.m....AAV packaging experiments. Let us know what you think about the calculator in the comments section below... do the math for you and it’s simple to make adjustments for the type of tissue culture dish and number... -
New Podcast Segment: Hot Plasmids
TypeBlog PostPublishedMay 17, 2017, 3:37 p.m.... Below, we break down each of the Hot Plasmids segments into its own (highly Tweetable!) audio clip. In...learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast Segment, or have...for new Hot Plasmids topics, let us know in the comments section below. Hot plasmids covered in this segment... -
Addgene’s Blog Gets a New Look!
TypeBlog PostPublishedJan. 25, 2021, 5:54 p.m....URL, same great articles to help you with your experiments, but a new look. We started the blog in 2013... community, share scientific discoveries and developments, and provide tips and tricks for new and seasoned...emailing us at [email protected] or tell us in the comments below. We’ll track down the story, get the inside... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog PostPublishedJune 15, 2016, 1:30 p.m....been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...teaching. He hopes to teach his molecular biology students about the functions of these powerful tools and...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community... -
Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up
TypeBlog PostPublishedApril 5, 2016, 2:30 p.m.... to carefully-screened research and teaching departments at universities around the world. Connecting ... assistance, and more to Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s...incredibly important for scientific breakthroughs, reagents, and know-how to transcend national borders and... -
Addgene’s Top 10 Blog Posts from 2014
TypeBlog PostPublishedDec. 16, 2014, 8:01 p.m....about their research, their job hunts, and their tips for experiments. We’ve also loved helping to answer... Plasmids 101 series and by responding to your comments. Additionally we’ve seen what topics are of interest... -
Fueled by Coffee at #SfN14
TypeBlog PostPublishedNov. 18, 2014, 7:15 p.m....fueled by highly caffeinated grad students and postdocs, which accounts for many, many lattes and espressos...infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have... -
2022 and 2023: Reflections and Looking Forward
TypeBlog PostPublishedFeb. 23, 2023, 2:15 p.m....’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...is used by the community to drive new science experiments. Finally, we were thrilled by how many people...from the expansion of our Data Hub to process improvements in every aspect of our organization. One thing... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m.... be used for A•T to G•C edits in protoplasts or plants of rice and wheat. Read the paper in Genome Biology...cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in diverse bacteria Carol Gross’s lab recently...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog PostPublishedJuly 7, 2017, 1:32 p.m....us to make organs using iPS cells derived from patients. Chapter 8: The importance of a mix of research... disease. How Darrell is using stem cells from patients to study various lung diseases. Chapter 10: How...and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity... -
Your Top Requested Plasmid in 2016!
TypeBlog PostPublishedJan. 11, 2017, 8:47 p.m.... strong as the basis for many genome editing experiments. So, without further ado, the most request plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols... -
Addgene's Top Blog Posts from 2020
TypeBlog PostPublishedDec. 29, 2020, 2:15 p.m....tools from depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV...began working with tech transfer offices to make reagents available to scientists in both academia and industry...Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...to get, as well as give, information to make improvements. Members connect directly with one another...in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many ... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m....competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...would be a wonderful tradition for university departments to establish, with the goal of fostering better...