...fueled by highly caffeinated grad students and postdocs, which accounts for many, many lattes and espressos...infiltration of CRISPR-Cas tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have...

...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...is used by the community to drive new science experiments. Finally, we were thrilled by how many people...from the expansion of our Data Hub to process improvements in every aspect of our organization. One thing...

...us to make organs using iPS cells derived from patients. Chapter 8: The importance of a mix of research... disease. How Darrell is using stem cells from patients to study various lung diseases. Chapter 10: How...and promotes open science in the lab - sharing reagents, cell lines, data, and knowledge without exclusivity...

... strong as the basis for many genome editing experiments. So, without further ado, the most request plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

... be used for A•T to G•C edits in protoplasts or plants of rice and wheat. Read the paper in Genome Biology...cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in diverse bacteria Carol Gross’s lab recently...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...tools from depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV...began working with tech transfer offices to make reagents available to scientists in both academia and industry...Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset...

...Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...to get, as well as give, information to make improvements. Members connect directly with one another...in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many ...

...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...would be a wonderful tradition for university departments to establish, with the goal of fostering better...

...At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade...which has already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has...you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually...

...This represents a considerable barrier to furthering research and developing effective treatments for ...dozens of wild-type genes and their associated mutants in other model systems. It is possible that a given...

...scientists quickly find much-needed phages for patients.   A phage directory is crucial because it's hard...Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, it's likely that...in the works. To keep up to date with the new developments at Phage Directory and in the phage field, subscribe...

...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...specific filter sets and experimental output requirements. Select from these yeast-optimized fluorophore...vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others may be interested...

...action - Search! Along with this we’ve also made improvements to our global navigation and menus over the ...built on the foundation of a community that shares reagents and knowledge with one another, and Addgene make...content above the fold, we decluttered the page elements and allowed our content to dictate the space. ...

...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...time. This made time for me to attend to other commitments, without feeling guilty about missing work. As...Northwestern University. She is one of several students completing their co-op at Addgene. This is her...

... A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies: A database... not listed in today’s post? Share them in the comments below. This post was written in April 2020 by ...protocols using apps to save you time and track your experiments  Resources on Addgene.org: Read our Molecular...

... use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing...two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than HcKCR2, making...long-sought tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer...

...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...-wide access to curated, high-quality data and reagents Addgene makes it easy for scientists to deposit...Twitter, Facebook, LinkedIn, email, or even the comments section of this post. We feel GREAT when you send...

...up the brightness in your fluorescent imaging experiments. Fluorescent protein fusions One of the easiest...Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In almost any...you to determine SunTag’s suitability in your experiments on a protein-by-protein basis. However, since...

...attractive imaging platform for in vivo imaging experiments because  ultrasound devices are inexpensive, ...coli strain Nissle 197 (ECN) was used for these experiments because it’s a probiotic strain that can colonize... and inflammatory bowel conditions. In these experiments, arg1 expressing ECN were injected into the mouse...