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We narrowed to 753 results for: Nes

Showing: 521 - 540 of 753 results

  2. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...transparency Another major benefit of preprints is their openness. Preprints can be read freely by anyone and are... work too openly too soon or simply a lack of awareness of the benefits of preprints. Some worry about...

  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...you cannot test them all, so how do you know which ones to try?  Read on and we will provide you with some...in knock-out or knock-down down tissues and cell lines; orthogonal strategies that compare antibody assay...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...methods are often significantly longer than direct ones.  Even though the secondary antibody incubation ...to improve direct methods and avoid the indirect ones. Some Western blots, for example, use a primary ...

  8. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    Published
    Jan. 29, 2015, 1:25 p.m.
    ...you through to a successful outcome and the good ones take this responsibility seriously. As I make my...Vivo Group whose tagline is Bridging Science and Business. Maybe you just need help getting through your...

  9. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...we’ve just presented some standard formatting guidelines for making a scientific poster, you shouldn’t...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... C. elegans embryo when all essential embryonic genes are individually depleted via RNA interference (...that can bind to activation sequences upstream of genes to stimulate expression (Wang et al., 2016). In ...

  11. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...with other measurements, specifically fluorescent ones? Many of the available biosensors use a single GFP...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...Since polyclonal antibodies contain a variety of clones and thus the potential to bind many different sites...secondary antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...genomes, scientists can control expression of their genes of interest. While recombinases are powerful gene... knockout library that targets human epigenetic genes was deposited by Kivanc Birsoy. Find the library...

  14. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency between...diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream of light...

  15. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...more I realized that it cuts across multiple disciplines, which really epitomizes what the makerspace ...Caenorhabditis Genetics Center (CGC) at the University of Minnesota has declared our makerspace an educational institution...

  16. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...provides the AAV replication (rep) and capsid (cap) genes. AAV replication uses the host’s polymerase, but... the pHelper plasmid which expresses adenovirus genes which help mediate AAV viral replication; and 3)...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...can be identified. Even if some mammalian cells lines have similar morphologies, being familiar with these...Twice a week often works for most fast-growing cell lines (such as HEK293, which multiplies every 16 hours...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...proteins both transiently or through stable cell lines. Both methods produce high protein yields if transfection...cells)  that allow for continuous expression of genes integrated into the insect cell genome. Both of ...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools for targeted...nuclease-competent Cas9, obviating the need to generate new cell lines or transgenic animals expressing dCas9 as a means...

  20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...any prime editor experimentalist, and especially shines once large-scale prime editing experiments come...
Showing: 521 - 540 of 753 results