We narrowed to 644 results for: Rest

1. Plasmids 101: NGS Quality Control for Pooled Libraries

   Type

   Blog Post

   Published

   Oct. 26, 2017, 1:59 p.m.

   ...and if you have any tips, input, or stories of interest about library use, let us know in the comments...

2. DAP arrays for multiplex gene editing

   Type

   Blog Post

   Published

   July 7, 2022, 1:15 p.m.

   ...editing, in virtually any cell type or organisms of interest with only minimal genetic payloads. Different ...

3. Addgene Welcomes John Doench to the Advisory Board

   Type

   Blog Post

   Published

   Aug. 13, 2015, 2:30 p.m.

   ...drawing me towards the Broad. In order to ask interesting biological questions, you need a tremendous amount...

4. Resume Writing for Non-academic Science Careers

   Type

   Blog Post

   Published

   Dec. 18, 2014, 4:06 p.m.

   ...browsing job postings and look at the jobs you are interested in. Consider listing the skills you already have...

5. Plasmids 101: Mammalian Vectors

   Type

   Blog Post

   Published

   March 25, 2014, 3:15 p.m.

   ...assayed conveniently. A direct test for your gene of interest may be another method to assess transfection success...

6. Fluorescent Proteins 101: Luciferases

   Type

   Blog Post

   Published

   July 22, 2025, 1:15 p.m.

   ...thousands of reporter constructs with a gene of interest already inserted. In addition, we have recently...

7. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...experiment.  For example, I used to constantly underestimate time needed to prep for an experiment (labeling...

8. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...

9. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...

10. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ... binds to a specific region of your protein of interest. For example, if you are studying a protein that...

11. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

12. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type

    Blog Post

    Published

    May 11, 2017, 2:30 p.m.

    ...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

13. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

14. Addgene’s Viral Service - Why Virus? Why Now?

    Type

    Blog Post

    Published

    March 23, 2017, 2:30 p.m.

    ... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

15. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References Højland...

16. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

17. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    Published

    Nov. 19, 2019, 2:08 p.m.

    ...ability to reversibly bind and release a protein of interest in response to light allows for tagless protein...

18. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology...

19. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

20. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...