We narrowed to 580 results for: SIM

1. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...

2. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...that facilitates HR What if promoting HR was as simple as swapping out your Cas9? It can be! Cas12a is...

3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...between Jan. 1 and Mar. 1, 2020. Figure 2: A simple approximation of the effect of COVID-19 on data...

4. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ...measure the concentration of multiple targets simultaneously. For AAV titration, you should only need to...

5. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...RNPs are capable of cleaving genomic targets with similar efficiency as compared to plasmid-based expression...

7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   Published

   Aug. 30, 2022, 1:15 p.m.

   ...mentioned above, organic solvents can be used to simultaneously fix and permeabilize a sample. But you can ...

8. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...or expertise from the start, or if you can begin simply and build up to more complexity if needed. And ...

9. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ... from fluorescent proteins given the relative simplicity of the system and the availability of fluorescent...

10. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ... 10 meters apart, are mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly...

11. A Career in Grants: How To Become a Grant Professional

    Type

    Blog Post

    Published

    Aug. 20, 2015, 2:30 p.m.

    ..., mid- to late-career grant professionals have similar stories about a time when their passion for an ...

12. Plasmids 101: Protein tags

    Type

    Blog Post

    Published

    Dec. 11, 2014, 4:26 p.m.

    ...blots, IP, and affinity purification. Arguably the simplest affinity tag is the polyhistidine (His) tag. Small...

13. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type

    Blog Post

    Published

    Dec. 2, 2014, 7:23 p.m.

    ... experiences in research labs. What are some similarities and differences you have observed as you have...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... the dsRed protein isolated from Discosoma sp. Similar work has been done to expand the red-fluorescent...

15. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...to be managing. Being reminded of the seemingly simple actions we can take to help ourselves can be an...

16. QC Sequencing Technologies at Addgene

    Type

    Blog Post

    Published

    July 15, 2025, 1:15 p.m.

    ...within Geneious.  While this may seem like it is simply repeating the steps that gave us our initial incorrect...

17. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse instead of...

18. Twenty Years of Sharing: Expanding Accessibility

    Type

    Blog Post

    Published

    Nov. 21, 2024, 10:47 p.m.

    ...about them. As early as 2007, we began grouping similar tools together in what would eventually become ...

19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...broadly useful vectors, a process that isn’t as simple as it seems. Take, for instance, the GCaMP-encoding...

20. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ...skills-based ones, may not use them very often. Similarly, entry-level jobs are less likely to require cover...