Skip to main content

We narrowed to 559 results for: ESP

Showing: 271 - 280 of 559 results
  1. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...
  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... and these are all symptoms of a negotiation, especially when money is involved. However, it doesn’t have...how much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...
  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... difficult to quantify accurately, but can be especially helpful to generate more signal from low abundance...the multicolor overlay; this helps all viewers, especially if you have some complex patterns with several...
  4. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied...expression, and can cross blood vessel barriers. It is especially popular for applications targeting liver tissue...
  5. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...recombinase expression may be replaced by sensors that correspond to the desired inputs. Such sensors do not necessarily...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...
  6. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...allows you to easily orient your gene insert with respect to the promoter. For example, let’s say your plasmid...being made (post or pre run in the table above, respectively). Some of the above stains require you to visualize...
  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...important to test it in the brain region of interest, especially when using DIO/FLEx vectors for off-target expression...relatively low recombination rate (0.1% and 0.13% respectively) still required a 200-300 fold dilution to minimize...
  8. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... lab Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware... my experience in the lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb ...
  9. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...considerations For changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher...efficiency, while for edits 3’ to the PAM sequences corresponding to the opposite strand were better (Farboud ...
Showing: 271 - 280 of 559 results