We narrowed to 835 results for: Eras

1. Sweating the Small Stuff: Details in the Lab

   Type

   Blog Post

   Published

   Feb. 8, 2022, 2:15 p.m.

   ...steps, or you rewrite the protocol and consolidate several steps into one. Soon you’ll figure out what prompts...

2. Early Career Researcher Toolbox: Social Media for Scientists

   Type

   Blog Post

   Published

   Dec. 11, 2018, 12:46 p.m.

   ...engineers on Twitter, but also led to interviews on Al Jazeera and NPR, articles in magazines like Fast Company...

3. How to Make Friends and Meet People at a Scientific Conference

   Type

   Blog Post

   Published

   Aug. 7, 2014, 1:58 p.m.

   ...socializing at a conference, but start with a more general intro until you know if the person you are talking...

4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

   Type

   Blog Post

   Published

   Feb. 18, 2014, 4:22 p.m.

   ... first meeting and get organized. Discuss your general areas of interest and what you want to cover during...

5. Will You Be My Mentor? Finding and Asking for Mentoring Support

   Type

   Blog Post

   Published

   Feb. 4, 2014, 3:50 p.m.

   ...relationships grow naturally out of friendships or work interactions. Watch for the people that are already teaching...

6. Seven Tips for Using LinkedIn as a Scientist

   Type

   Blog Post

   Published

   Oct. 1, 2019, 1:21 p.m.

   ...like a police photo if you look straight at the camera. Pro tip: Turn off notifications to your network...

7. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in...

8. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...

9. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

   Published

   June 16, 2022, 1:15 p.m.

   ...it takes, ooh, approximately the length of your average PhD to achieve!) Each field has its own framework...

10. Addgene: The First Twenty Years

    Type

    Blog Post

    Published

    Dec. 19, 2024, 2:15 p.m.

    ...channel in 2013, which continue to grow today. “I generally refer people to Addgene for the basics of molecular...

11. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type

    Blog Post

    Published

    May 11, 2021, 1:15 p.m.

    ...interest. However, we also found that this is not generalizable to all fusion proteins. For example, we saw ...

12. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ...MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans...

13. Lab Automation at Addgene

    Type

    Blog Post

    Published

    March 22, 2018, 1:28 p.m.

    ...Label your tubes with a label maker. Label makers generate far more legible labels than your hand and help...

14. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...can spread between hosts through horizontal (or “lateral”) gene transfer. Horizontal gene transfer can occur...

15. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...calcium sensors for in vivo studies) There are several calcium reporters specially designed for the aims...

16. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...

17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type

    Blog Post

    Published

    Feb. 23, 2016, 3:30 p.m.

    ...homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in the PITCh vector ~20 bp 5’...

18. Plasmids 101: Multicistronic Vectors

    Type

    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry...

19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... PARIS, a zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary...

20. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...you probably need to find a new job. If you are miserable at home or work, your relationships will suffer...

21. Plasmids 101: Codon usage bias

    Type

    Blog Post

    Published

    Sept. 27, 2018, 1:09 p.m.

    ...of protein known as inclusion bodies that will generally segregate with the cell pellet during purification...

22. Plasmids 101: Shuttle Vectors

    Type

    Blog Post

    Published

    May 20, 2025, 1:15 p.m.

    ...experiment? Not quite. Shuttle vectors have to address several big differences in the ways vectors are used across...

23. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...setup. We recommend starting by searching the literature to see what is already known about your housekeeping...

24. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    Published

    Aug. 23, 2016, 2:30 p.m.

    ... care about these details, but does follow the general structure and flow of the entire session. I myself...

25. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...Excitation Laser Color Very Bright Bright Moderate Dim Ultraviolet (355 nm)   BD Horizon...