We narrowed to 842 results for: Eras

...recombinase-dependent vectors. We recently released several recombinases in various serotypes, and will continue...

...proof-of-concept experiments for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from...

... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...

...Voigt’s lab used this system to develop a bacterial camera that prints a chemical image, as well as a genetic...

...steps, or you rewrite the protocol and consolidate several steps into one. Soon you’ll figure out what prompts...

...sequencing results. At Addgene, we now use next-generation sequencing during our QC process. This way, we...

...and holds promise in the development of future therapies. Our popular plasmid encoding a Cre dependent ...

...across the majority of cellular pathways. Despite several years of research, the mechanisms of miRNA regulation...

...engineers on Twitter, but also led to interviews on Al Jazeera and NPR, articles in magazines like Fast Company...

...socializing at a conference, but start with a more general intro until you know if the person you are talking...

... first meeting and get organized. Discuss your general areas of interest and what you want to cover during...

...relationships grow naturally out of friendships or work interactions. Watch for the people that are already teaching...

...it takes, ooh, approximately the length of your average PhD to achieve!) Each field has its own framework...

...interest. However, we also found that this is not generalizable to all fusion proteins. For example, we saw ...

...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in...

...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...

...channel in 2013, which continue to grow today. “I generally refer people to Addgene for the basics of molecular...

...like a police photo if you look straight at the camera. Pro tip: Turn off notifications to your network...

...Label your tubes with a label maker. Label makers generate far more legible labels than your hand and help...

...calcium sensors for in vivo studies) There are several calcium reporters specially designed for the aims...

...and goal of the experiment. Now, onto the tips! General principles pegRNA design If you’re just starting...

...can spread between hosts through horizontal (or “lateral”) gene transfer. Horizontal gene transfer can occur...

...MicroPublication 1 (2018): 2–3. Wang, Han, et al. "cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans...

...homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in the PITCh vector ~20 bp 5’...

...Retroviral pEF1a-IRES-Neo IRES Mammalian MSCV-IRES-Luciferase IRES Retroviral pWPI IRES Lentiviral AmCyan-P2A-mCherry...

... PARIS, a zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary...

...you probably need to find a new job. If you are miserable at home or work, your relationships will suffer...

...experiment? Not quite. Shuttle vectors have to address several big differences in the ways vectors are used across...

...of protein known as inclusion bodies that will generally segregate with the cell pellet during purification...

...setup. We recommend starting by searching the literature to see what is already known about your housekeeping...