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  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ...switches were designed as a genetic tool for researchers to conditionally manipulate gene expression in...allowing for expression of GeneR. This allows a researcher to test whether or not expressing GeneR alone...

  2. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...Eric Green, Director of the National Human Genome Research Institute (NHGRI) of the NIH, started off with...acid science still has many surprises for the researchers in this field. Resources at Addgene Browse ...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...FRET based tools to study epigenetics Epigenetic research uses a wide range of molecular biologic techniques...none of these techniques can be used for in-situ research of dynamic modifications in real-time, posing ...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... the battle when using optogentics to answer a research question. The flip side of the optogenetics coin... molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures.2011.01.007...

  5. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ... of a gene exists, there must be assays that researchers can use to quantify said copies. They do so using...commonly available and may not be an option to all researchers. Accuracy needed Do you need to call exact...

  6. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...set out to fill this gap by highlighting that researchers learn many transferable skills while working ...

  7. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid library encompassing...encapsulating a single enzyme. Compared with previous research, which required protein compartments to co-localize...

  8. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    ...bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss Institute for Biologically Inspired...University of California, Santa Cruz, where her research is focused on engineering nanoparticles as drug...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...resistance gene can affect a cell's phenotype, researchers usually remove it after selection using the Cre...conditional knockout. To make a conditional knockout, researchers often use the Cre/Lox system described earlier...

  10. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...of CRISPR-based immune systems in bacteria and archaea. Class 1 is further broken down into different ...ubiquitous in nature, they are largely underutilized in research settings — mostly a consequence of Cascade's complexity...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...recent studies (Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted...Newby, G. A., Raguram, A., & Liu, D. R. (2019). Search-and-replace genome editing without double-strand...

  12. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...has a research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician...

  14. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    Published
    Jan. 30, 2018, 2:42 p.m.
    ... A recent survey of PhDs found that many researchers feel that they lack formal training in a variety...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working...

  15. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...This post was updated on March 21, 2018. Most of the time, plasmid prepping is a breeze. You get your...copy number of the empty plasmid backbone or a researcher’s personal observations. If you are experiencing...

  16. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...al.’s use of MMEJ represents another strategy researchers can exploit for CRISPR gene editing. In organisms...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented detail...A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2) e0001-15.2015. Pubmed. Resources...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...strategies most widely incorporated into plasmids for research purposes are described below. IRES Elements Translation...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  20. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...structure (Figure 1). Here, MXS Chaining gave researchers the ability to generate the four initial unique...acid. The Journal of biological chemistry. 1961. March;236:864–875. Pubmed PMID: 13790780. 5. Shetty RP...
Showing: 541 - 560 of 777 results