Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 799 results for: Des

Showing: 541 - 560 of 799 results

  1. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...seeking “public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

  2. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...The natural CRISPR locus of a bacteria host encodes multiple guide RNAs (gRNAs) on a single array to ...applications, we provide detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715...

  3. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...visiting role is because the host lab or institute provides something that you can’t obtain at your current...skills and equipment! Preparing for your visit   Design and communicate a research plan Your time at the...

  4. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...biomass production techniques in the following decades, Phillips made the decision to release the K. phaffii...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...effects, both of happen with Cas13. CRISPR-Csm degrades both nuclear and cytoplasmic mRNA with 90-99% ...crystalizing the hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you...

  6. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...if you end up concentrating your AAV above your desired titer and want to dilute it, err on the side of...less buffer than you would to achieve the exact desired concentration to avoid over-diluting, and make ...

  7. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...form concatemers in later steps. This new NEB kit includes a step that both fragments the DNA and prevents...of the workshop, NEB gave a brief presentation describing the technology. Then we split into pairs to actually...

  8. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... determine if proteins are targeted to their destination co- or post-translationally. TRICK: Visualizing... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

  10. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... Science. For each publication, Web of Science provides basic bibliographic information and uses its vast...citations due to the journal of publication. Table 1 provides an example calculation of the weights. Table 1...

  11. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing". Dr. Yamanaka...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ...

  12. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...mammalian plasmid components, it may be useful to describe the means of introducing genetic material (such...the plasmid in the same way. Selection methods, described below, are typically employed when creating stable...

  13. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ..., the use of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning...the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...

  14. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...familiar with the basics of controls in experimental design, you’ll need a few controls specific to flow as...controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls...

  15. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... such as citizenship status, academic year, and grades that serve as automated filters of consideration...and cover letter revised by your network, which includes your friends, family members, and professional...

  17. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...1x1012 vg/mouse is more standard) and reflects a desire for very high MOI on top of very high coverage....researchers have used the flexibility of AAV genome design in remarkably creative ways. The continued development...

  18. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes also ...well as the genes that encode those proteins are designated by the lower-case Greek letters μ, δ, γ, ε, and...

  19. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...promoter should flank this component as well. On both sides of this promoter-gene combo are two long terminal...Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from: https://www.ncbi.nlm.nih.gov/books...

  20. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation...reagents accessible to your lab. The solution GCE provides almost endless possibilities for researchers, ...
Showing: 541 - 560 of 799 results