We narrowed to 593 results for: ELP

1. Simplify Cloning with in vivo Assembly

   Type

   Blog Post

   Published

   Oct. 18, 2018, 12:37 p.m.

   ...sequence manager, such as SnapGene, can be very helpful to confirm primer design.  Use minimal template...

2. Better Dyeing Through Chemistry & Small Molecule Fluorophores

   Type

   Blog Post

   Published

   Sept. 8, 2016, 2:30 p.m.

   ...Brett Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke...

3. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

   Type

   Blog Post

   Published

   March 17, 2016, 2:30 p.m.

   ...between labs, initial titration experiments might be helpful to achieve optimal results. It is important to ...

4. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Oct. 14, 2015, 2:30 p.m.

   ...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...

5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...constructs like plasmids often include GFP as a marker to help identify which cells have successfully taken up ...

6. Transferable Skills Guide: Career Planning Resources

   Type

   Blog Post

   Published

   Nov. 14, 2017, 2 p.m.

   ...offering advice on areas where you might need some help. Today: Career Planning Resources. The key to ensuring...

7. Fluorescent Proteins 101: Aptamer Fluorophores

   Type

   Blog Post

   Published

   April 11, 2017, 2:30 p.m.

   ...problematic, however. Well designed aptamers can help reduce such background, thereby improving traditional...

8. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ...option in such instances is palliative care, which helps patients manage the pain and discomfort caused by...

9. Viral Production at Addgene

   Type

   Blog Post

   Published

   Oct. 24, 2023, 1:15 p.m.

   ...serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol...

10. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...have any questions, don’t hesitate to email us at help@addgene.org. Happy screening! This blog was written...

11. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker...

12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type

    Blog Post

    Published

    July 7, 2020, 1:15 p.m.

    ...created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated...

13. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...Shintani et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids...

14. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    ...AAG to improve editing efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient...

15. Antibodies 101: The Four ELISAs and When to Use Them

    Type

    Blog Post

    Published

    July 25, 2024, 1:15 p.m.

    ...target is not expressed. The negative control will help you determine if there is non-specific binding or...

16. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ... brand new location, with more space to grow and help scientists share resources in new ways. Along with...

17. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ...analyzed. While the many different ELNs out there can help with searchability, record-keeping, and data sharing...

18. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ...necessary or for long-term experiments. They’ve also helped researchers design and optimize cloning methodologies...

19. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ...Our internal software was originally designed to help us work in large formats, such as 96-well microplates...

20. Antibodies 101: Immunoprecipitation

    Type

    Blog Post

    Published

    Dec. 7, 2021, 2:15 p.m.

    ...overview of the IP method. If you found this blog helpful be sure to check out Addgene’s blog for other antibody...