We narrowed to 600 results for: ELP

...approaches you with glowing feedback from a client you helped. Maybe you awkwardly say thanks and move on with...

...in the guide across from these off-target edits helped to minimize local off-target editing by RESCUE....

...Fabienne Furt, and Quintin Marcelino for their helpful discussion of this book....

...sequence manager, such as SnapGene, can be very helpful to confirm primer design.  Use minimal template...

...to be aware of and actively use tools designed to help reduce or eliminate bias, just like the ones made...

...Brett Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke...

...between labs, initial titration experiments might be helpful to achieve optimal results. It is important to ...

...Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which are difficult...

...constructs like plasmids often include GFP as a marker to help identify which cells have successfully taken up ...

...overall size), using TnpB as the core nuclease could help keep the total construct size smaller than Cas9 ...

...highlight sequence identities in problem areas, helping us pinpoint the issue and resolve the assembly....

...AAG to improve editing efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient...

...target is not expressed. The negative control will help you determine if there is non-specific binding or...

...serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences. AAV preparations are purified by iodixanol...

...have any questions, don’t hesitate to email us at help@addgene.org. Happy screening! This blog was written...

...offering advice on areas where you might need some help. Today: Career Planning Resources. The key to ensuring...

...piggyBac vector integrates into genomic DNA with the help of the piggyBa transposase. Image from Hazelbaker...

...created an array of base editing reporters that can help you do just that. Monitoring APOBEC and Cas9-mediated...

...Shintani et al., 2015). Now, bioinformatics tools help researchers determine how compatible their plasmids...

...overview of the IP method. If you found this blog helpful be sure to check out Addgene’s blog for other antibody...