We narrowed to 556 results for: ESC;

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

   Type

   Blog Post

   Published

   May 13, 2025, 1:15 p.m.

   ...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

2. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

3. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

4. Deep Mutational Scanning with One Pot Saturation Mutagenesis

   Type

   Blog Post

   Published

   Feb. 22, 2017, 3:30 p.m.

   ... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

5. Multiple Plasmids at a Low Price: Kits from Addgene

   Type

   Blog Post

   Published

   Sept. 14, 2016, 2:30 p.m.

   ...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

6. Treating Muscular Dystrophy with CRISPR Gene Editing

   Type

   Blog Post

   Published

   Jan. 26, 2016, 3:30 p.m.

   ...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

7. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   Published

   Sept. 1, 2015, 2:30 p.m.

   ...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

8. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...

9. CRISPR 101: Epigenetics and Editing the Epigenome

   Type

   Blog Post

   Published

   June 24, 2020, 5:45 p.m.

   ...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

10. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

11. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural...

12. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    Published

    July 6, 2021, 1:28 p.m.

    ...genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution...

13. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    Published

    Feb. 25, 2020, 2:15 p.m.

    ...3rd year graduate student in the lab of Prof. Francesco Paesani at University of California San Diego....

14. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    Published

    May 14, 2024, 1:15 p.m.

    ...are designed for flexibility. The Fragmid kit described above works in both mammalian systems and Drosophila...

15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...Fact! Even though the Cas9:RNA complex is well described in the closed conformation, recent electron microscopy...

16. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type

    Blog Post

    Published

    Jan. 13, 2025, 9:49 p.m.

    ...’s first demonstration of prime editing used to rescue a genetic disease phenotype in an animal model,...