...nucleotide insertions, dele­tions or other mutations, referred to as ‘indels’. While this is convenient for generating... iPSCs (or correct them back to wild type), differentiate them into somatic cells affected by the disease...iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones! We ...developing novel iPSC-based model systems.     References 1. Cong, Le, et al. "Multiplex genome engineering...

...of interest supports a variety of processes in different cell types. One great way to do this is to use...SuperPLD module is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes...future updates to the collection! Resources and references Additional resources on the Addgene blog Viral....org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan, N., Ononiwu...

...insertions, like a fluorescent protein knock-in, suffer from low efficiency when a standard dsDNA repair... secondary structure. Today, multiple companies offer long single-stranded DNA production services or ...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice...

...directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ...into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or, to avoid handling embryos ex ...therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib...

...TALENs more specificity and a broader substrate preference than the ZFNs.   Genetic Engineering Era: Post...genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie version (2016) stars Tom Hanks...that are typically associated with the DSBs and offered unprecedented prospects of curing diseases caused...your research Browse plasmids for base editing References Anzalone AV, Randolph PB, Davis JR, Sousa AA,...

...nutrient-rich and also the best source for mycoplasma proliferation. Plus, it is added to the media after autoclaving... contact with a nutrient source, they start proliferating shortly. Once they are present in the laboratory...Bioluminescence assay kits are available from different biotech companies which are enzyme-based mycoplasma...and a Bachelor's degree in Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous...

... away from the bench, I have worked for seven different organizations in a variety of science policy roles...cultural, and political. Science policy looks differently depending on how and where you pursue it. It ...you do science policy? Just like there are many different types of science policy careers, there are many...NSPN maintains a great resource guide including conferences, societies, and outreach groups. Grab a virtual...

...Addgene is excited to announce we now offer an export license application for rabies materials! Some ...rabies plasmids to international requestors. We also offer export license applications for VSV and SARS-COV...before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application...

...search engines, which allow you to search many different antibody catalogs at the same time.  If you're...Library for Cancer Research Human Luekocyte Differentiation Antigen Workshops Immune cells Flow cytometry...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any ...

...has deposited If you’d like to write about a different topic, feel free let us know. What do you get ...audience of biological scientists from a variety of different fields. Exposure - With over 100,000 views each...interact with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning...

...and the importance of realizing that different people have different management needs. How Crystal developed...and deciphering job listings. Mobility between different positions at Novartis. Chapter 7: How Crystal ...

...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways...Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal... Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde...

...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality... Jennifer Tsang...

... been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...only better for the environment, but most likely safer for researchers, as well. Additional resources...

...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...of the students were interested to see so many different career options in science, with Abigail Anderson...professor. There are amazing opportunities that are offered with a biology major.” After hearing about many...

... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...

...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...ultrasound dyes because these dyes “collapse” at different ultrasound frequencies. Read more about ‘Using...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter...