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We narrowed to 895 results for: Ide

Showing: 541 - 560 of 895 results

  1. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...efficiencies and expression levels in the cells. Ideally, the chosen reporter is unique to the cell, is ... generations. Stably transfected cells may be considered an entirely new cell line from that of the original..., NIH3T3, CHO, 293HEK, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation 100...hygB HeLa, NIH3T3, CHO, Jurkat T cells Blocks polypeptide synthesis at 80S; inhibits chain elongation. ... 10-14 days depending on how quickly your cells divide. Gentamicin is often used as a supplement in mammalian...

  2. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...fall back to? We have outlined some important considerations to help you choose the plasmid prep kit that...or by incorporating recycled paper and plastic. Aside from the more obvious swaps like using smaller bottles...Where can you look for help?  All manufacturers provide specs, protocols, and quality control information...help explain the rationale behind each step and provide troubleshooting advice. In these cases, it can ...between these manufacturers, as price is a large consideration for most labs. Many institutions have preferred...department for these.  While budget is an important consideration, you do want to make sure you’re getting the...

  3. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...protein. Animals are first injected with a small peptide that represents a short, unique amino acid sequence...isolate individual hybridomas and ELISA is used to identify the hybridomas producing the most specific antibodies...antibody, meaning that the antibodies are largely identical between lots.  Disadvantages of monoclonal antibodies...antibody-encoding genes over time as the hybridoma cells divide. These changes can alter the antibody that is actually...produced by the hybridoma. Any genetic drive can be identified by sequencing the heavy and light chains. To ...

  4. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...of dNTP in the reaction mix, the researcher can decide exactly how far the T4 exonuclease proceeds: at...at the first occurrence of the chosen nucleotide, T4 will switch to polymerizing and then stall due to...the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...multi-fragment DNA assembly, and its low cost makes it ideal for researchers doing large amounts of cloning. ... 10.1007/978-1-61779-564-0_5. PubMed. JBEI-J5’s guide to SLIC Resources at Addgene Ligation-Independent...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...particular marker, you can use the reference plots provided by the antibody companies or check the literature...sample. The isotype antibody will be virtually identical to the antibody for your marker of interest. It...for that particular marker. This enables you to identify any non-specific binding to know that your positive...unsure what amount of antibody to use, you should consider using an isotype control. Pro tip! Many companies...Cytometry Resources on Addgene.org Addgene's Antibody Guide Browse Addgene's Antibody Collection        ...

  6. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    Published
    June 25, 2019, 12:54 p.m.
    ...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...inject their genetic material into the cell. Once inside, phages can follow one of two different life cycles...both of these life cycles bacterial DNA can be accidentally packaged into the newly created phages. Transfer...referred to as transduction. Transferred DNA once inside the infected bacterium can either exist as transient...Addgene’s protocol page, which has both protocols and videos for techniques in basic molecular biology, plasmid...

  7. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus Guide Page ...understand brain function So far scientists have identified 180 brain regions. Both this geography and the...Using retrograde AAV AAVrg can be used to deliver a wide array of genetic tools for neural interrogation ...where the cell bodies of the projection neurons reside.   Impact for scientists While the mechanisms ...

  8. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...suggests a change to a slide, think about whether the same principle applies to other slides in your deck.  If... craft sentences for abstracts and presentation slides, learn from audiences as they do science outreach...of a new science communication initiative that provides yet more scientists with opportunities to practice...Resources on Addgene.org Check out how Addgene uses videos for science communication ...

  9. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    Published
    Nov. 19, 2019, 2:08 p.m.
    ...more OptoNBs would allow for more uses both inside and outside cells. References Gil, Agnieszka A., et...exciting because using light to control signaling provides greater spatiotemporal resolution than pharmacological...domain of the Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed...more about optogenetics in Addgene's optogenetics guide ...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    .... To make a point mutation, you design two oligonucleotide primers with the desired mutation, then use...3-4 phage proteins are needed and these can be provided via a separate plasmid or be inserted into the...insertions and deletions. The figure above shows guidelines for primer design; a desired insertion should...REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion size, primer...introduce mutations. Using commercially available high-fidelity polymerases such as KOD and Phusion, which are...

  11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    ... in the human genome. The pair has focused considerable attention on transcription factors, starting...have good antibodies. The results look almost identical. Addgene: What advantages does your system have... also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges... Myers: Only a small percentage of the proteins inside a cell have a commercial antibody against them.... to us to get them. Even though we’re happy to provide them, that never works on a large scale. The world...

  12. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...to $47,476 per year. This post discusses how a nationwide injunction against the FLSA is affecting universities...injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe that...higher than the level being raised to now - across a wide consensus of academic stakeholders. However, while...collecting data on the effects of the injunction nationwide and, at exactly one month following the injunction...plans, but have since reversed their position and decided to allow individual departments to go ahead with...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...time-consuming aspects of traditional cloning is the identification of clones that actually contain your insert...enzyme-mediated disruption of ccdB, leading to positive identification of desired clones.   ...Becomes a powerful... insert of interest. Hence correct clones are identified much more efficiently, as those that do not contain...ultimately perform the same function, there is some evidence that ccdB Survival™ can be more difficult to transform...E. coli do not contain the F plasmid (and are considered F-); however, there are a few popular lab strains...

  14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...variant characteristics they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage...researchers also showed that Archon1 is functional in a wide range of animal models such as mice, C.elegans, ...display technique using small scaffold proteins to identify novel binding proteins that bind to either the...because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools were also...

  15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...tumors may be the stars, there's plenty of action outside of tumor cells, and recent studies (Martins et ...microenvironments made of?  A tumor’s genesis is divided into 3 phases: initiation, progression, and metastasis...to the development and spread of cancer cells. Besides these stromal cell modifications, stromal cells...transportation, exosomes are tools of communication and provide malignancy to the microenvironment itself. They...malignancy, concluding that these cells may be considered "natural tumor suppressors." A growing interest...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...impact.  {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing...wonder if providing a viral vector service was a good idea. The scientific community is loving it. Almost 10,000...about this, but definitely not worried. I have confidence that from Business Development to Shipping we...

  17. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz ...administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory experiments...therefore the transduced gene is episomal and does not divide and replicate in the same way the host DNA does...Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources...

  18. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...thousands of plasmids, using both mass production genome wide screens and the more traditional “one at a time”...time. We also handle the MTA, QC the plasmids, provide helpful resources, and, on the rare occasion things...organized lab saves time and money across the board. Consider alternative cloning strategies There are many ...either don’t scale or have limited scaling. The downside of high-throughput work is that you give up a ...engagement with and use of repositories like Addgene provides large-scale savings. How large? Using Doench’s...

  19. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...affects protein function (Bornhorst 2000).  One downside of polyhistidine tag-based purification, however...contamination, such as mammalian production systems, consider using these more selective substrates along with... containing a polyhistidine tag, you’ll need to decide whether to add the tag to the N- or C-terminus ...the opposite end.  If neither placement works, consider purifying under denaturing conditions. Unlike ...References  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources...

  20. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    Published
    Feb. 2, 2018, 3:20 p.m.
    ...even come [to grad school]!” He asked if she had considered other careers, which she hadn’t.  He then said... didn’t know if she liked science writing. She decided to start a blog to test it out and discovered she...communications planning.” To beef up her resume, she decided to complete a certificate in Strategic Communications...these difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills...important for her work include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things...
Showing: 541 - 560 of 895 results