We narrowed to 716 results for: Met

...vectors. While viral delivery is a highly effective method for gene editing and allows targeting of specific...annealed oligos into the digested vector using your method of choice. Step 5: Colony Screening Use Primer...

...enzymes (BsaI, BbsI/BpiI, BsmBI), which recognize asymmetric DNA sequences and cleave at a short distance ...stocks beforehand. You can also use high-throughput methods to streamline your plasmid extractions. Use this...

..., then the other, a dual-enrichment and mass spectrometry workflow identifies proteins bearing both labels... of proximity labeling in living cells. Nature Methods, 20(6), 908–917. doi: 10.1038/s41592-023-01880-...

..., or pretty much anyone applying the scientific method to a question. Videos from any discipline are welcome...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

...goal posts” as a part of a case, consulting can sometimes be challenging. The fast paced nature of projects...of great output in a short amount of time can sometimes become cauldrons on the verge of boiling. On other...

...Tends to resist promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...Obstacles for CRISPR HIV-1 therapy Both of these methods represent exciting advances in preclinical HIV-...

...equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all the major equipment...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...

... body works, generating new therapeutics, and sometimes formulating new biological concepts. However, ... technique simply by following the abbreviated methods section found within a published manuscript. Luckily...

...milestone for the molecular biologist was to devise methods to manipulate it. The term genetic engineering ...history of CRISPR Read about other genome editing methods Resources on Addgene.org Visit our CRISPR guide...

...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...universal scoring system for selecting a gRNA, as the method of producing the guide (synthetic, in vitro transcription...

... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...

...through multiple cell divisions, indicating that this method produces stable edits. However, this system is ... effects, ABEs also compare favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified...

...immunohistochemistry, immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher...

.... If you’re looking to design your first flow cytometry experiment, “Chapter 7: Sorting” is there to help...

...optogenetics tools for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR...

...McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips for Getting a Faculty ...

... people in a better mood. Laughter itself can sometimes even count as demanding exercise, and, of course...

...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick...

... Chew's lab. Genome engineering, gene delivery methods, AAVs, and more. Chapter 7/Outro: Advice for researchers...