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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 711 results for: Met

Showing: 541 - 560 of 711 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ..., or pretty much anyone applying the scientific method to a question. Videos from any discipline are welcome...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...Tends to resist promoter down regulation due to methylation or deacetylation. Ubc General expression mRNA... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...reservoirs, chronic inflammation, and negative metabolic effects of ART. Why can’t the body mount an immune...Obstacles for CRISPR HIV-1 therapy Both of these methods represent exciting advances in preclinical HIV-...

  4. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying all the major equipment...their success. Therefore, assume positive intent. Sometimes, “no we don’t have the funds,” really means they...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...technology, and the user may need to optimize numerous parameters to generate the desired edit. The wrench: Gene...universal scoring system for selecting a gRNA, as the method of producing the guide (synthetic, in vitro transcription...

  7. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...milestone for the molecular biologist was to devise methods to manipulate it. The term genetic engineering ...history of CRISPR Read about other genome editing methods Resources on Addgene.org Visit our CRISPR guide...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... constructs that compose genetic circuits and metabolic pathways. For many MoClo systems, the individual...interested in recombinant protein purification and metabolic engineering. If instead you want to build genetic...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...through multiple cell divisions, indicating that this method produces stable edits. However, this system is ... effects, ABEs also compare favorably to other methods. In a head-to-head comparison with Cas9, Cas9 modified...

  10. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...immunohistochemistry, immunocytochemistry, and flow cytometry. It's a great introduction to — or quick refresher...

  15. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this quick...

  20. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ...
Showing: 541 - 560 of 711 results