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We narrowed to 850 results for: NTS

Showing: 541 - 560 of 850 results

  2. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...scientists quickly find much-needed phages for patients.   A phage directory is crucial because it's hard...Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, it's likely that...in the works. To keep up to date with the new developments at Phage Directory and in the phage field, subscribe...

  3. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade...which has already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has...you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins) and therefore usually...

  4. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

  5. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...specific filter sets and experimental output requirements. Select from these yeast-optimized fluorophore...vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others may be interested...

  6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...action - Search! Along with this we’ve also made improvements to our global navigation and menus over the ...built on the foundation of a community that shares reagents and knowledge with one another, and Addgene make...content above the fold, we decluttered the page elements and allowed our content to dictate the space. ...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...time. This made time for me to attend to other commitments, without feeling guilty about missing work. As...Northwestern University. She is one of several students completing their co-op at Addgene. This is her...

  8. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...studies rely on delivering reversible or varied amounts of stimulation to receptors. Optogenetics offers...deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR If precise spatiotemporal control of...brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan Roth lab for feedback on...

  9. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...of your sample over time and determine which experiments you can effectively use the DNA. pH and DNases...protein degradation, and/or the use of chelating agents that can be introduced in the purification protocol...without having substantial degradation and which experiments you can use your plasmid.  DNA elution choices...

  10. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...attractive imaging platform for in vivo imaging experiments because  ultrasound devices are inexpensive, ...coli strain Nissle 197 (ECN) was used for these experiments because it’s a probiotic strain that can colonize... and inflammatory bowel conditions. In these experiments, arg1 expressing ECN were injected into the mouse...

  11. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

  12. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ... A data repository for validation data on IBEX reagents.  mAb3D atlas validated antibodies: A database... not listed in today’s post? Share them in the comments below. This post was written in April 2020 by ...protocols using apps to save you time and track your experiments  Resources on Addgene.org: Read our Molecular...

  13. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...unpublished materials. What about preprints? Addgene supports preprints and encourages authors to share ...preprint, or without any paper at all. Posting preprints and openly sharing materials are both excellent...

  14. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...resources continuously raising money from donations or grants to fulfill their mission. Addgene is fortunate ...-wide access to curated, high-quality data and reagents Addgene makes it easy for scientists to deposit...Twitter, Facebook, LinkedIn, email, or even the comments section of this post. We feel GREAT when you send...

  15. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...up the brightness in your fluorescent imaging experiments. Fluorescent protein fusions One of the easiest...Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In almost any...you to determine SunTag’s suitability in your experiments on a protein-by-protein basis. However, since...

  16. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...biological sciences and constitute foundational elements of biomedical research. Traditional restriction...purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning...directly into chemically competent E. coli and transformants selected with the appropriate antibiotic. Although...

  17. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ... use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons, far out-performing...two-photon excitation around 1,040 nm, has larger photocurrents, and is more selective for K+ than HcKCR2, making...long-sought tool for researchers to control K+ gradients and can be used as a potent optogenetic silencer...

  18. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... variety in their job. Good relationships with clients and colleagues Sometimes this means you have to... in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be the...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...

  20. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...
Showing: 541 - 560 of 850 results