...studies rely on delivering reversible or varied amounts of stimulation to receptors. Optogenetics offers...deschloroclozapine (DCZ) has been developed to meet these requirements. TL;DR If precise spatiotemporal control of...brainDissecting circuits Studying GPCR pathways   Acknowledgements We thank the Bryan Roth lab for feedback on...

...of your sample over time and determine which experiments you can effectively use the DNA. pH and DNases...protein degradation, and/or the use of chelating agents that can be introduced in the purification protocol...without having substantial degradation and which experiments you can use your plasmid.  DNA elution choices...

... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...editing plasmids for mammalian systems, bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and...

...biological sciences and constitute foundational elements of biomedical research. Traditional restriction...purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning...directly into chemically competent E. coli and transformants selected with the appropriate antibiotic. Although...

... variety in their job. Good relationships with clients and colleagues Sometimes this means you have to... in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be the...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...

...hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ...

...RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains...

...reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that...

...in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...

...find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy?  Contact...

... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...

...of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and...

...candidate transcript targets analyzed. CRISPR-Csm presents an attractive alternative for nuclear-RNA targeting...hfYFP structure, the group rationally designed GFP variants with similar capabilities. If you’re looking for... temperatures, and in the presence of oxidizing agents. Adapted from Fig. 2 Campbell et al, Nature Methods...

...Once you’ve identified some fly stocks and other reagents of interest, the next question to ask is what ...background. In other cases, YFG or one of its mutant variants will need to be overexpressed or knocked down....Although it’s often very easy to get GAL4 UAS experiments up and running with fly lines that are already...

...relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis... their paths cross with each other and with the events of the times."     - Aliyah Weinstein, Marketing...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

...Recombinases And more! Control AAV For many AAV experiments, we recommend validating the serotype in your...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific... should improve the specificity of influence measurements by reducing photostimulation of the axons and...

...a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were... factors developed thus far for these latter experiments, such as transcription factors tethered to dCas9...

...cells (as demonstrated in different cellular environments such as retina or glioblastoma). They also found...classical purification protocol involving iodixanol gradients. The current purification protocols for isolating...decipher the mechanistic aspects behind these improvements. What have exosome AAVs achieved so far? Exo-AAVs...

...high-security facility needed to perform the experiments to demonstrate that this approach was useful ...diverse. Some of them are undergraduates or PhD students who are just starting their journey into science...stops these one-time favors from becoming starting points for long-lasting partnerships Join us! We are a...