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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 753 results for: Nes

Showing: 541 - 560 of 753 results

  1. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...other colonies containing smaller, recombined backbones that may replicate faster. For this reason, if...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  2. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...CRISPR plasmids categorized by year of deposit. Outlines show a representative proportion of CRISPR plasmids...of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is both enduring and ...

  3. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing. The more I talked...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

  4. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...fewer transcripts of their antibiotic resistance genes and can therefore be cultured in media containing.... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ... the hierarchy of web pages the plasmid page is nested in. You can see in the example above that the bread...Database has more information about common vector backbones.  The backbone modifications listed can refer ...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin ... might not, however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation...

  8. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...protocols and examples of applications from across disciplines now available. In this post, we’ll review the...example, cytoskeletal structures that form continuous lines in pre-expanded samples can sometimes take on a ...

  9. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...Japan, of course, it is optimized for living for Japanese, which is not trivial, and the grant situation...eager to interact, which I don't think is good for Japanese science. I really miss the intellectual environment...

  10. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...variety of chromogenic, chemifluorescent, and chemiluminescent substrates are available. Labs with limited...reader as opposed to chemifluorescent, and chemiluminescent methods that require specialized equipment...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level... cover letter should be no more than a page. Conciseness is key! If possible, address to the professor...

  13. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ... than optimal user experience and the overall soundness of the software design. Each of us brings slightly...Demo details for this would be something along the lines of showing that the checkout takes the user directly...

  14. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...easier. You should also demonstrate a strong willingness to help others with their problems, a general...sorts of details about the application, just not the ones that matter. Therefore, if there is a bit of a Sherlock...

  15. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...base pairs long and found upstream of their target genes. The sequence of the promoter region controls the...elements, insulators, and silencers; however, some "leakiness" of transcription may occur. This is normally ...

  16. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...need to keep paper records as well as electronic ones? It is important to know in which ways your data...functionalities such as: electronic signatures, electronic witnessing and audit trails. How does the ELN provider ...

  18. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...development, extended-open integrins enable tissue morphogenesis, organ development, and the shaping of the extracellular...the scientists and scientific process behind the scenes to the new antibodies and research coming out of...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...constructs for targeting Golgi, ER, and endosome membranes) (Figure 4). The authors used SuperPLD to manipulate...illumination, substantial PA accumulates in the plasma membranes, indicated by the PA probe colocalizing with SuperPLDx30...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...rules) to some volunteers, who filmed with their phones. Test: I loved the end result, but it was clear....  Build: If you’ve ever watched any behind the scenes footage in the last several years, you can usually...
Showing: 541 - 560 of 753 results