We narrowed to 818 results for: TIM

...tracing of the performance of each gRNA multiple times within the same experiment by counting both the ...restriction enzyme digestion of single molecules in real-time. Find plasmids expressing tTALE-FP Shin et al., ...

... that could be transferred between cells. At the time, these extranuclear agents of heredity were thought...the widely adopted name for these elements. At the time, the use of episome seemed appropriate, especially...

...described in 1971,” McSweeney said. “But, it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012... working on several genetic diseases at the same time. The limitation of budget restrained us from costly...

...compete against each other. Getting two-color stimulation has been a holy grail of the field. The problem...to try these optogenetic tools out for the first time? Boyden: There are always considerations for each...

... four variants of these biosensors, which are optimized for specific parameters: jGCaMP7s is the most...specificity of influence measurements by reducing photostimulation of the axons and dendrites of non-targeted...

...understanding of PZA action and could potentially stimulate the development of useful diagnostic methods and...non-linear microscopy methods will be used to stimulate and manipulate neural populations in order to ...

...reproducibility because mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying...are verified and identifiable to save researchers time and make their work more reproducible. While Addgene...

...playing with some cards and I had a quick flashback to time spent playing a card game called “Magic”. There ...certain target cell population. Who is the winner? Ultimately, society should be! This is a collaborative game...

...body and how it works. I used to stay up past my bedtime, poring over my grandparents’ medical textbooks...communication as a PhD student Fortunately, by the time I was ready to graduate with my BSc in Anatomy, ...

...scientists of all areas who volunteered to donate their time and expertise to help them. In less than two weeks...and easily credited. And nothing stops these one-time favors from becoming starting points for long-lasting...

...voltage indicators (GEVIs) change fluorescence in real time when neurons or muscle cells transmit electrical... and conditions that can be used to monitor real time voltage changes in worms and have made these constructs...

... folder that you are currently working in) every time you use them, leave them in their own folder. Doing...Organized Learn about Software You Can Use to Save Time and Track Your Experiments Try out the CRISPR Software...

...Once upon a time, not so long ago, spCas9 was the only Cas enzyme widely available and applied by researchers...better experimental fit due to this. But now, it’s time to cast aside those concerns and embrace a new resource...

...bogged down with distributing them and spending more time on research. What do you hope Addgene will accomplish...and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. Additional...

...transgenic flies to study your favorite gene (YFG).  Sometimes, you want to use the Gal4/UAS system, but the ... as the intestines, can be dissected at various times throughout the lifespan of the fly to determine ...

...perform under your experimental conditions. This is time well spent and, if you do it right, can be (part...is calculated by multiplying EC by QY (EC*QY, sometimes normalized to the value of EGFP). The higher the...

... and develop skill in science communication over time.  Like most skills, great science communication ... to pose these why questions to yourself, but ultimately there is no substitute for feedback from others...

...How many times have you looked at a diagram depicting transcription, or DNA repair, or replication, or... Berkley Gryder. Addgene depositors or not, your time is valuable ...

...a talk about the different parts of the brain stimulated by stories. In retrospect, I should have expected...looking to get into the field, this is a fantastic time to do it! Additional Resources on the Addgene Blog...

... of Cas9 expression between experiments and over time (as compared to separate transductions with a single-vector...(Gi)-mCherry When using this virus for the first time, the Roth Lab recommends verifying cre expression...